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| Status |
Public on Dec 10, 2011 |
| Title |
Expression data from mouse cumulus cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Follicle stimulating hormone (FSH) and epidermal growth factor (EGF) are currently used on cumulus-oocyte complexes to mimic the luteinizing hormone surge in vitro and induce oocyte maturation and cumulus expansion. We have previously shown that addition of exogenous recombinant growth differentiation factor 9 (GDF9) during oocyte in vitro maturation led to an improvement of oocyte quality, as shown by an increased blastocyst percentage and fetal survival.
Our objective was to characterize the effect of FSH/EGF and GDF9 treatments on mouse cumulus cells expression profile by microarray analysis.
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| Overall design |
Cumulus-oocyte complexes (COCs) were recovered from 21 to 26 day old female 129/SV mice, 44 hours post equine chorionic gonadotropin treatment (eCG (5 IU)). For the microarray experiment whole COCs were treated with 293H control medium (0.125% v/v), with 20 ng/ml GDF9 or with a combination of 50 mIU/ml FSH and 10 ng/ml EGF. After 8 hours of in vitro maturation, COCs were denuded by gentle pipetting, the oocytes were removed and the cumulus cells centrifuged and extracted RNA analysed by microarray.
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| Contributor(s) |
Sasseville M, McDowall M, Thompson JG, Gilchrist RB |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 04, 2009 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Maxime Sasseville |
| E-mail(s) |
Maximesasseville@hotmail.com
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| Organization name |
The Robinson Institute, University of Adelaide
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| Street address |
Level 6, Medical school, Frome Road
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| City |
Adelaide |
| State/province |
SA |
| ZIP/Postal code |
5005 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM480233 |
Mouse Cumulus_8hours_293Hcontrol_rep1 |
| GSM480234 |
Mouse Cumulus_8hours_293Hcontrol_rep2 |
| GSM480235 |
Mouse Cumulus_8hours_293Hcontrol_rep3 |
| GSM480236 |
Mouse Cumulus_8hours_293Hcontrol_rep4 |
| GSM480237 |
Mouse Cumulus_8hours_GDF9-treated_rep1 |
| GSM480238 |
Mouse Cumulus_8hours_GDF9-treated_rep2 |
| GSM480239 |
Mouse Cumulus_8hours_GDF9-treated_rep3 |
| GSM480240 |
Mouse Cumulus_8hours_GDF9-treated_rep4 |
| GSM480241 |
Mouse Cumulus_8hours_FSH/EGF-treated+293Hcontrol_rep1 |
| GSM480242 |
Mouse Cumulus_8hours_FSH/EGF-treated+293Hcontrol_rep2 |
| GSM480243 |
Mouse Cumulus_8hours_FSH/EGF-treated+293Hcontrol_rep3 |
| GSM480244 |
Mouse Cumulus_8hours_FSH/EGF-treated+293Hcontrol_rep4 |
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| Relations |
| BioProject |
PRJNA120425 |
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