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| Status |
Public on May 24, 2022 |
| Title |
PRD class homeobox genes in bovine early embryos: function, evolution and overlapping roles |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
ETCHbox genes are mammalian-specific PRD class homeobox genes with conserved expression in the preimplantation embryo but fast-evolving and highly divergent sequences. We ectopically expressed bovine ARGFX and LEUTX ETCHbox genes, both tagged with 3xFLAG tags, in bovine foetal fibroblasts and completed transcriptome seqencing. We compared the transcriptome of cells with ARGFX or LEUTX ectopic expression with control transfected cells. For ARGFX, we expressed the WT version of the gene and a mutant allele containing a 13bp frameshift deletion.
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| Overall design |
Ectopic expression of bovine ARGFX and LEUTX homeobox genes in bovine foetal fibroblasts followed by transcriptome sequencing. 4 conditions (control, WT ARGFX, mutant ARGFX, LEUTX, each with 3 or 4 biological replicates).
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| Contributor(s) |
Lewin TD, Fouladi-Nashta AA, Holland PW |
| Citation(s) |
35512670 |
| Submission date |
Dec 21, 2021 |
| Last update date |
May 24, 2022 |
| Contact name |
Thomas Lewin Lewin |
| E-mail(s) |
thomas.lewin2@wolfson.ox.ac.uk
|
| Organization name |
Thomas Lewin
|
| Department |
Department of Biology
|
| Street address |
11a Mansfield Road
|
| City |
Oxford |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX1 3SZ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA791233 |
| SRA |
SRP351865 |
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