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| Status |
Public on Dec 10, 2004 |
| Title |
Identification of PDGF-dependent patterns of gene expression in U87 glioblastoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Overall study: Identification of PDGF-dependent patterns of gene expression in U87 glioblastoma cells.
RNA was obtained from triplicate dishes of 5 different groups of U87 cells, each (total 15) analyzed with one U95 microarray chip.
Three different comparisons were made:
1) Clone 3.1 (34580-34582) vs. clone 3.3 (34583-34585) vs. parent U87 (34592-34594).
Purpose: demonstrate that the gene expression profiles between these 3 cell lines are not different, so they could be pooled as a single untreated group.
2) Pooled control group (34580-34585, 34592-34594) vs. clone 8.1 (34586-34588).
Purpose: identify genes specifically controlled by autocrine PDGF activity.
3) Clone 8.1 (34586-34588)
vs. clone 8.1 treated with PDGF (34589-34591)
Purpose: Identify genes specifically induced by exogenous PDGF.
Keywords = platelet-derived growth factor
Keywords = glioblastoma
Keywords = brain cancer
Keywords = sterol regulatory element binding protein
Keywords = SREBP
Keywords: ordered
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| Contributor(s) |
Ma D, Nutt C, Shanehsaz P, Peng X, Louis DN, Kaetzel DM |
| Citation(s) |
15994924 |
| Submission date |
Nov 03, 2004 |
| Last update date |
Dec 13, 2018 |
| Contact name |
David Murray Kaetzel |
| E-mail(s) |
dmkaetz@uky.edu
|
| Phone |
859-257-6558
|
| Fax |
859-323-1981
|
| Organization name |
University of Kentucky College of Medicine
|
| Department |
Molecular and Biomedical Pharmacology
|
| Lab |
Cancer Division
|
| Street address |
800 Rose Street
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40536-0298 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
|
| Samples (15)
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| Relations |
| BioProject |
PRJNA90949 |
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