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| Status |
Public on Oct 31, 2009 |
| Title |
Expression data of influenza A infected human macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human disease caused by highly pathogenic avian influenza (HPAI) H5N1 can lead to a rapidly progressive viral pneumonia leading to acute respiratory distress syndrome. There is increasing evidence suggests a role for virus-induced cytokine dysregulation in contributing to the pathogenesis of human H5N1 disease. The key target cells for the virus in the lung are the alveolar epithelium and alveolar macrophages, and previous data has shown that compared to seasonal human influenza viruses, equivalent infecting doses of H5N1 viruses markedly up-regulate pro-inflammatory cytokines in both primary cell types in vitro. The dysregulation of H5N1-induced host responses is therefore important for understanding the viral pathogenesis.
We used microarrays to analyze and compare the gene expression profiles in primary human macrophages after influenza A virus infection.
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| Overall design |
Peripheral-blood leucocytes were separated from buffy coats of three healthy blood donors and cells were differentiated for 14 days before use. Differentiated macrophages were infected with H1N1 and H5N1 at a multiplicity of infection (MOI) of two. Total RNA was extracted from cells after 1, 3, and 6h post-infection, and gene expression profiling was performed using an Affymetrix Human Gene 1.0 ST microarray platform.
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| Contributor(s) |
Lee SM, Gardy JL, Cheung C, Cheung TK, Hui KP, Ip NY, Guan Y, Hancock RE, Peiris JM |
| Citation(s) |
20011590 |
| Submission date |
Oct 30, 2009 |
| Last update date |
Jul 26, 2018 |
| Contact name |
SMY Lee |
| Organization name |
The University of Hong Kong
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| Street address |
21 Sassoon Road
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| City |
Hong Kong |
| ZIP/Postal code |
HKG |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (27)
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| GSM466570 |
donor 1, mock infected macrophages at 1h |
| GSM466571 |
donor 1, H1N1 infected macrophages at 1h |
| GSM466572 |
donor 1, H5N1 infected macrophages at 1h |
| GSM466573 |
donor 1, mock infected macrophages at 3h |
| GSM466574 |
donor 1, H1N1 infected macrophages at 3h |
| GSM466575 |
donor 1, H5N1 infected macrophages at 3h |
| GSM466576 |
donor 1, mock infected macrophages at 6h |
| GSM466577 |
donor 1, H1N1 infected macrophages at 6h |
| GSM466578 |
donor 1, H5N1 infected macrophages at 6h |
| GSM466579 |
donor 2, mock infected macrophages at 1h |
| GSM466580 |
donor 2, H1N1 infected macrophages at 1h |
| GSM466581 |
donor 2, H5N1 infected macrophages at 1h |
| GSM466582 |
donor 2, mock infected macrophages at 3h |
| GSM466583 |
donor 2, H1N1 infected macrophages at 3h |
| GSM466584 |
donor 2, H5N1 infected macrophages at 3h |
| GSM466585 |
donor 2, mock infected macrophages at 6h |
| GSM466586 |
donor 2, H1N1 infected macrophages at 6h |
| GSM466587 |
donor 2, H5N1 infected macrophages at 6h |
| GSM466588 |
donor 3, mock infected macrophages at 1h |
| GSM466589 |
donor 3, H1N1 infected macrophages at 1h |
| GSM466590 |
donor 3, H5N1 infected macrophages at 1h |
| GSM466591 |
donor 3, mock infected macrophages at 3h |
| GSM466592 |
donor 3, H1N1 infected macrophages at 3h |
| GSM466593 |
donor 3, H5N1 infected macrophages at 3h |
| GSM466594 |
donor 3, mock infected macrophages at 6h |
| GSM466595 |
donor 3, H1N1 infected macrophages at 6h |
| GSM466596 |
donor 3, H5N1 infected macrophages at 6h |
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| Relations |
| BioProject |
PRJNA121239 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18816_RAW.tar |
103.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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