|
| Status |
Public on Nov 10, 2022 |
| Title |
COPD and non-diseased nasopharyngeal and bronchial organoids characterization by single cell RNA-seq |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Clinical COPD, characterised by intermittent and infective exacerbations, lacks cellular model systems for the study of host-pathogen relationships. We establish nasopharyngeal and bronchial organoids from COPD patients and healthy individuals. In contrast to healthy organoids, COPD organoids demonstrate the hallmark goblet cell hyperplasia phenotype with reduced ciliary beat frequency, leading to impaired mucociliary clearance. By single-cell transcriptome analysis, smooth trajectory of cellular differentiation is disrupted in COPD organoids when compared to non-diseased, and functional pathways involved in the development and progression of COPD including mitochondrial dysfunction and sirtuin signalling are activated in the COPD model.
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| |
|
| Overall design |
Characterization of lung organoids derived from healthy and COPD donors for cell population, differentiation process and pathway analysis
|
| |
|
| Contributor(s) |
Chan LL, Ivan FX, Cheng HS, Tan NS, Chotirmall SH |
| Citation(s) |
36496442, 37963864 |
| Submission date |
Oct 15, 2021 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Fransiskus Xaverius Ivan |
| E-mail(s) |
fivan@ntu.edu.sg
|
| Organization name |
Nanyang Technological University
|
| Department |
Lee Kong Chian School of Medicine
|
| Street address |
11 Mandalay Road
|
| City |
Singapore |
| ZIP/Postal code |
308232 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA771701 |
| SRA |
SRP341634 |
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