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| Status |
Public on Nov 24, 2009 |
| Title |
Drosophila melanogaster spermatogenesis expression profile: mitotic, meiotic and post-meiotic cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
We conducted a genome-wide expression analysis of wild-type males using three cell populations isolated from mitotic, meiotic and post-meiotic phases of spermatogenesis in Drosophila melanogaster. Our approach was to directly isolate testis regions enriched with RNAs from each of the three specific germline phases.
We used microarrays to detail the global gene expression profile in spermatogenesis and identified up- and down-regulated genes between two different spermatogenic phases in pairwise comparisons
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| Overall design |
Cell types present at various stages of spermatogenesis are generally located in a gradient along the proximal-distal axis of the testis, however most are not exclusively restricted to any one geographic region. Cells enriched for mitotic, meiotic and post-meiotic phases were obtained by dissection of apical, proximal and distal regions of the testis, respectively
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| Contributor(s) |
Vibranovski MD, Lopes HF, Karr TL, Long M |
| Citation(s) |
19936020 |
| Submission date |
Oct 09, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Maria D Vibranovski |
| E-mail(s) |
mdv@uchicago.edu
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| Phone |
17738343567
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| Fax |
17737029740
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| Organization name |
University of Chicago
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| Department |
Ecology and Evolution
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| Lab |
Dr. Manyuan Long
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| Street address |
1101 East 57th Street
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (9)
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| GSM461118 |
Male mitotic cells, biological rep1 |
| GSM461119 |
Male mitotic cells, biological rep2 |
| GSM461120 |
Male mitotic cells, biological rep3 |
| GSM461121 |
Male meiotic cells, biological rep1 |
| GSM461122 |
Male meiotic cells, biological rep2 |
| GSM461123 |
Male meiotic cells, biological rep3 |
| GSM461124 |
Male post-meiotic cells, biological rep1 |
| GSM461125 |
Male post-meiotic cells, biological rep2 |
| GSM461126 |
Male post-meiotic cells, biological rep3 |
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| Relations |
| BioProject |
PRJNA120251 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18502_RAW.tar |
19.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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