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| Status |
Public on Oct 01, 2013 |
| Title |
The effects of Hmgb1 depletion on gene expression in murine cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
High Mobility Group Box 1 (HMGB1) is a highly abundant and evolutionarily conserved non-histone chromatin component with the ability to bind and bend DNA. The protein is involved in fundamental nuclear processes including nucleosome sliding, transcription, replication, V(D)J recombination and DNA transposition. To assess the functional impact of HMGB1 on transcription we performed gene expression profile analyses of mouse embryonic fibroblasts, wild-type or knockout for the Hmgb1 gene
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| Overall design |
2 genetic backgrounds: wild-type (Wt), Hmgb1-/- (KO), 3 biological replicates (rep1, rep2, rep3; S phase synchronized cells in duplicate only), no technical replicates. Each pair of wt and Hmgb1-/- MEFs was isolated from embryos born from a single Hmgb1+/- mother crossed with a Hmgb1 +/- male. Total RNAs from three pairs of MEFs, derived from three different mothers and cultured up to passage 3, were extracted using mirVana miRNA Isolation Kit (Ambion) and analyzed on the Illumina BeadsArray platform.
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| Contributor(s) |
Celona B, Agresti A, Bianchi ME, Rossetti G, Rossi RL, Pagani M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 07, 2009 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Riccardo Lorenzo Rossi |
| E-mail(s) |
rossi@ingm.it
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| URL |
http://www.ingm.it
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| Organization name |
Fondazione INGM
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| Department |
Integrative Biology
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| Lab |
Pagani
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| Street address |
Via Trivulzio 10
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| City |
Milano |
| State/province |
MI |
| ZIP/Postal code |
20149 |
| Country |
Italy |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA118187 |
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