|
| Status |
Public on Apr 01, 2022 |
| Title |
Single-cell profiling of histone modifications and transcription in developmental systems with EpiDamID [mouse EB] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We developed EpiDamID, an adaptation of existing DamID protocols that extends their applicability to histone PTMs.
|
| |
|
| Overall design |
Profiling H3K27me3 and Ring1B in single-cells of Embryonic Bodies at day 7, 10 and 14 days post aggregation with scDam&T-seq.
|
| |
|
| Contributor(s) |
Rang FJ, de Luca KL, de Vries SS, Boele E, Valdes-Quezada C, Nguyen PD, Guerreiro I, Sato Y, Kimura H, Bakkers J, Kind J |
| Citation(s) |
35366395 |
| Submission date |
Sep 13, 2021 |
| Last update date |
Jun 24, 2022 |
| Contact name |
Jop Kind |
| E-mail(s) |
j.kind@hubrecht.eu
|
| Organization name |
Hubrecht Institute
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| ZIP/Postal code |
3584CT |
| Country |
Netherlands |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL30172 |
NextSeq 2000 (Mus musculus) |
|
| Samples (18)
|
| GSM5576309 |
DamH3K27me3 - EB and ESC day07 (KIN5599_index09) |
| GSM5576310 |
DamH3K27me3 - EB and ESC day07 (KIN5599_index10) |
| GSM5576311 |
DamH3K27me3 - EB and ESC day10 (KIN5599_index13) |
| GSM5576312 |
DamH3K27me3 - EB and ESC day14 (KIN5599_index05) |
| GSM5576313 |
DamH3K27me3 DamOnly DamRing1B - EB and ESC day07 (KIN5434_index31) |
| GSM5576314 |
DamH3K27me3 DamOnly DamRing1B - EB and ESC day10 (KIN5434_index32) |
| GSM5576315 |
DamH3K27me3 DamOnly DamRing1B - EB and ESC day14 (KIN5434_index33) |
| GSM5576316 |
DamH3K27me3 DamOnly DamRing1B - EB and ESC day14 (KIN5599_index02) |
| GSM5576317 |
DamOnly - EB and ESC day07 (KIN5599_index11) |
| GSM5576318 |
DamOnly - EB and ESC day10 (KIN5434_index15) |
| GSM5576319 |
DamOnly - EB and ESC day14 (KIN5599_index06) |
| GSM5576320 |
DamRing1B - EB and ESC day07 (KIN5578_index14) |
| GSM5576321 |
DamRing1B - EB day07 (KIN5156_index25) |
| GSM5576322 |
DamRing1B - EB day07 (KIN5600_index13) |
| GSM5576323 |
DamRing1B - EB and ESC day10 (KIN5599_index12) |
| GSM5576324 |
DamRing1B - EB and ESC day14 (KIN5599_index04) |
| GSM5576325 |
DamRing1B - EB day14 (KIN5156_index27) |
| GSM5576326 |
DamRing1B - EB day14 (KIN5156_index42) |
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| This SubSeries is part of SuperSeries: |
| GSE184036 |
Single-cell profiling of histone modifications and transcription in developmental systems with EpiDamID |
|
| Relations |
| BioProject |
PRJNA763006 |
| SRA |
SRP337336 |
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