|
| Status |
Public on Oct 07, 2004 |
| Title |
EWS-FLI1-transfection of HEK293 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Response of HEK293-cells after transfection with EWS-FLI1. HEK293 cells were transfected with the expression vector pIRES2-EGFP containing type I EWS-FLI1 or empty control vector. For transient transfection standard DEAE dextran method was used and RNA was isolated 48h post transfection. For stable transfection cells were transfected using FuGENE 6 (Roche, Mannheim, Germany) and cells were selected with 400 ug/mL G418. DNA-microarray analysis was performed using Affymetrix HG-U133A microarrays.(see Staege et al. Cancer Res. 2004)
Keywords = HEK293
Keywords = EWS-FLI1
Keywords = Ewing family tumors
Keywords: other
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| Contributor(s) |
Staege MS, Hutter C, Neumann I, Foja S, Hattenhorst UE, Hansen G, Afar D, Burdach SE |
| Citation(s) |
15548687 |
| Submission date |
Oct 05, 2004 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Christoph Hutter |
| E-mail(s) |
christoph.hutter@medizin.uni-halle.de
|
| Phone |
+49-345-5577265
|
| Fax |
+49-345-5577275
|
| URL |
http://www1.medizin.uni-halle.de/paediatrie/
|
| Organization name |
Martin-Luther-University Halle-Wittenberg
|
| Department |
Universitätsklinik u. Poliklinik für Kinder- u. Jugendmedizin
|
| Lab |
Prof. Dr. S.E.G. Burdach
|
| Street address |
Weinbergweg 22
|
| City |
Halle (Saale) |
| State/province |
Sachsen-Anhalt |
| ZIP/Postal code |
06120 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (9)
|
| GSM31808 |
HEK293 stably EWS-FLI1-transfected, clone |
| GSM31809 |
HEK293 transiently EWS-FLI1-transfected 1 |
| GSM31810 |
HEK293 transiently EWS-FLI1-transfected 2 |
| GSM31811 |
HEK293 stably pIRES2-EGFP-transfected |
| GSM31812 |
HEK293 transiently pIRES2-EGFP-transfected 1 |
| GSM31813 |
HEK293 transiently pIRES2-EGFP-transfected 2 |
|
| Relations |
| BioProject |
PRJNA90505 |
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