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| Status |
Public on Aug 11, 2009 |
| Title |
Thrombospondin-1: A Pro-Atherosclerotic Protein Augmented by Hyperglycemia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Diabetes is associated with a more aggressive form of atherosclerosis. Thrombospondin-1 (TSP-1), an extracellular matrix protein, is an acute phase reactant that induces vascular smooth muscle (VSMC) migration and proliferation in areas of vascular injury, and is also upregulated in VSMCs exposed to hyperglycemia. We hypothesized that hyperglycemia amplifies the expression of genes induced by TSP-1 in VSMCs. Human aortic VSMCs were cultured in DMEM supplemented with 10 % FBS and 1% antibiotics, cells were used between passages three and five. VSMCs were preincubated in DMEM containing 0.2% FBS with 5 mM glucose (normoglycemia), 25 mM glucose (hyperglycemia), 25 mM mannose (osmotic control), TSP-1 (20µg/mL), 25 mM glucose + TSP-1 (20µg/mL) or 25 mM mannose + TSP-1 (20µg/mL). Data analysis revealed that TSP-1 stimulates gene expression relevant to the pathogenesis of atherosclerosis and diabetic vascular disease.
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| Overall design |
Total RNA was extracted from replicate cultures of human aortic VSMCs and microarray analysis performed for a total of 18 samples (3 replicates per condition) using the Human Gene Array ST.
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| Contributor(s) |
Maier KG, Han X, Sadowitz B, Gentile KL, Middleton FA, Gahtan V |
| Citation(s) |
20299182 |
| Submission date |
Aug 07, 2009 |
| Last update date |
Apr 25, 2019 |
| Contact name |
Frank A Middleton |
| E-mail(s) |
middletf@upstate.edu
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| Phone |
315-464-7721
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| Organization name |
SUNY Upstate Medical Univ
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| Department |
Neuroscience and Physiology
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| Street address |
505 Irving Avenue
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| City |
Syracuse |
| State/province |
NY |
| ZIP/Postal code |
13210 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA118655 |
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