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| Status |
Public on Apr 23, 2024 |
| Title |
Human Clariom S Array in OVCAR8 cells after silencing HSD17B14 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We used a functional small interfering RNA (siRNA) screen in ovarian cancer cells to identify 17β-hydroxysteroid dehydrogenase type 14 (HSD17B14) as a novel regulator of small EV secretion.
We showed that the knockdown of HSD17B14 altered multiple gene expression in OVCAR8 cells.
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| Overall design |
OVCAR8 cells were transfected with siControl and siHD17B14 for 48 hours. Total RNA was isolated using TRizol reagent (Thermo Fisher Scientific) and Direct-zol column kit (Genesee Scientific).
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| Contributor(s) |
Ma S, Court KA, Sood AK, Ivan C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 25, 2021 |
| Last update date |
Apr 23, 2024 |
| Contact name |
Shaolin Ma |
| Organization name |
MD Anderson Cancer Center
|
| Street address |
1881 East Road
|
| City |
Houston |
| State/province |
Tx |
| ZIP/Postal code |
77054 |
| Country |
USA |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA724979 |
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