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Status |
Public on Dec 24, 2010 |
Title |
Human NTERA2 (NT2/D1) cells lines: shZRF1 vs. shRandom during Retinoic Acid (RA) administration |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Transcriptional profiling of human NT2 cell lines comparing control cells (shRandom) with ZRF1 knockdown cells (shZRF1) both either untreated (0 h) or treated with Retinoic Acid (3 h) at 0.01 µM. The cell lines were established by retroviral infection allowing for the transcription of either a random shRNA (shRandom) or a shRNA specific for ZRF1 (shZRF1). The goal was to determine the effect of ZRF1 on transcriptional activation at the onset of differentiation.
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Overall design |
Four-condition experiment, shRandom vs. shZRF1 cells either uninduced (0 h) or RA induced (3 h). Biological replicates: 6 replicates (shRandom) taken on 3 different days without or with RA administration. 6 shZRF1 replicates taken on three different days with or without RA administration.
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Contributor(s) |
Richly H, Lopez-Bigas N, Di Croce L |
Citation(s) |
21179169 |
Submission date |
Jun 26, 2009 |
Last update date |
Jan 23, 2019 |
Contact name |
Luciano Di Croce |
E-mail(s) |
luciano.dicroce@crg.eu
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Phone |
+34933160132
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Organization name |
Centre for Genomic Regulation
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Street address |
Dr. Aiguader, 88
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City |
Barcelona |
ZIP/Postal code |
08003 |
Country |
Spain |
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Platforms (1) |
GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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Samples (12)
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GSM422425 |
shZRF1 vs shRandom 0h Replicate 1 |
GSM422426 |
shZRF1 vs shRandom 0h replicate 1 dye swap |
GSM422427 |
shZRF1 vs shRandom 0h Replicate 2 |
GSM422428 |
shZRF1 vs shRandom 0h replicate 2 dye swap |
GSM422429 |
shZRF1 vs shRandom 0h Replicate 3 |
GSM422430 |
shZRF1 vs shRandom 0h replicate 3 dye swap |
GSM422431 |
shZRF1 vs shRandom 3h Replicate 1 |
GSM422432 |
shZRF1 vs shRandom 3h replicate 1 dye swap |
GSM422433 |
shZRF1 vs shRandom 3h Replicate 2 |
GSM422434 |
shZRF1 vs shRandom 3h replicate 2 dye swap |
GSM422435 |
shZRF1 vs shRandom 3h Replicate 3 |
GSM422436 |
shZRF1 vs shRandom 3h replicate 3 dye swap |
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This SubSeries is part of SuperSeries: |
GSE16891 |
Human NTERA2 (NT2/D1) cells lines |
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Relations |
BioProject |
PRJNA122657 |