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| Status |
Public on Jan 27, 2021 |
| Title |
Genome-wide analysis of PGC-1α-binding in microglia |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
PGC-1α in microglia protects against ischemia-induced brain damage in mice. The data suggest that microglia-specific PGC-1α play a key role in limiting ischemia-induced brain damage and potently participates in regulating microglial function. To further clarify the mechanism of PGC-1α, we conducted chromatin immunoprecipitation-sequencing (ChIP-Seq) analysis to identify the targets of PGC-1α in microglia from mPGC-1α mice at 24 h after ischemic stroke. KEGG pathway analysis of these genes identified the mitophagy signaling pathway as one of the most highly enriched pathways. Finally, we demonstrated that PGC-1α induces mitophagy by regulating ULK1 expression in an ERRα-dependent manner, thereby reducing neuroinflammatory reactions.
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| Overall design |
Primary microglia were isolated from mPGC-1α mice at 24 h after ischemic stroke using the MojoSortTM mouse P2RY12 selection kit (Biolegend, San Diego, CA, USA) according to the manufacturer’s protocol. Then the samples were processed to perform ChIP-Seq analysis.
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| Contributor(s) |
Han B, Jiang W, Hao J |
| Citation(s) |
33771213 |
| Submission date |
Jan 26, 2021 |
| Last update date |
Apr 05, 2021 |
| Contact name |
Bin Han |
| E-mail(s) |
hanbin1128@163.com
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| Organization name |
Tianjin Medical University General Hospital
|
| Street address |
154 Anshan Road, Heping District
|
| City |
Tianjin |
| ZIP/Postal code |
300052 |
| Country |
China |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA694972 |
| SRA |
SRP303353 |
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