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| Status |
Public on Sep 06, 2022 |
| Title |
OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [ATAC-Seq] |
| Organisms |
Gallus gallus; Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The transcription factors OCT4 and SOX2 play an essential role in the establishment and maintenance of pluripotent embryonic stem cells (ESCs). Yet, their function in specialized stem cell populations is still poorly understood. Here, we show that the OCT4 and SOX2 work as dimers to regulate the epigenomic landscape of neural crest cells. By isolating primary neural crest cells at a range of developmental stages, we characterized the transcriptomic and epigenomic changes that take place during specification, migration, and early differentiation. Analysis of these datasets revealed that the OCT4/SOX2 dimer promotes an epigenomic signature inherent to the multipotent neural crest. We found that the emergence of this epigenomic state requires the translocation of OCT4/SOX2 to tissue-specific cis-regulatory regions. By examining genome organization during the induction of hESCs into neural crest cells, we observed that the patterns of genomic occupancy of the dimer are modified during cell fate commitment. Dimer translocation is guided by neural crest-specific pioneer transcription factors, which physically interact with the OCT4/SOX to modify their genomic targets. Our results demonstrate how the ESC pluripotency program is repurposed in specialized stem cells to control chromatin organization and define the developmental potential of embryonic progenitors.
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| Overall design |
There are three separate experiments in which we performed OMNI-ATAC-Seq. Group #1 - We electroporated chicken embryos with a TFAP2AE1-driven GFP reporter, which marks neural crest. We isolated GFP+ neural crest cells via FACS from various stages across early chicken development, and subjected them to OMNI-ATAC-Seq. Group #2 - We electroporated chicken embryos bilaterally. One side received a control vector (TFAP2AE1-GFP + empty PCI-H2B-RFP) and the other recieved an overexpression vector (TFAP2AE1-GFP + PCI-OCT4-SOX2-H2B-RFP). We isolated GFP+/RFP+ cells from the control and treatment side of the embryos, and performed OMNI-ATAC-Seq on each sample. Group #3 - We generated induced neural crest (iNCCs) from hESC. Along their differentiation, we performed OMNI-ATAC-Seq at multiple timepoints.
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| Contributor(s) |
Hovland AS, Bhattacharya D, Rothstein M, Simoes-Costa M |
| Citation(s) |
36182685 |
| Submission date |
Dec 23, 2020 |
| Last update date |
Oct 28, 2022 |
| Contact name |
Marcos Simoes-Costa |
| E-mail(s) |
simoescosta@cornell.edu
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| Organization name |
Cornell University
|
| Department |
Molecular Biology and Genetics
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| Lab |
Simoes-Costa Lab
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| Street address |
526 Campus Rd
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| City |
Ithaca |
| State/province |
New York |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (2) |
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| Samples (26)
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| GSM4986881 |
11306_10331_109901_HG3JFBGXC_TGGTCA_HH10_1 |
| GSM4986882 |
11419_10946_112321_HVKM5BGXC_ACAGTGGT_HH10_2 |
| GSM4986883 |
11419_10946_112321_HVKM5BGXC_CAGATCCA_HH12_1 |
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| This SubSeries is part of SuperSeries: |
| GSE163961 |
OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency |
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| Relations |
| BioProject |
PRJNA687499 |
| SRA |
SRP299102 |
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