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| Status |
Public on Dec 06, 2020 |
| Title |
Comparison of gene expression in CD8 T cells treated with the agonist of PPAR isoforms |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We performed gene expresion analysis of T cell treated with agonist of three isoform of PPAR. This analysis allowed us to explain why the activation of PPAR g is the best in boosting PD-1 blockade efficacy compared with other isoform activation.
We demonstrated that activation of the PPAR-γ isoform specifically enhanced PD-1 blockade greater than activation of the other two PPAR isoforms (a and b) in mouse tumor model. Using the microarray assay we found genes that were most differentially regulated by PPAR agonist treatment were with a well-documented role in T cell biology, such as Ddx3y, Ifng, Il17c, Ccr3, Acrv1, Btk and metabolic process such as Pygl, P2rx1, Tph2, Kynu, and Ugt2b37. Therefore, microarray data has been useful to establish that activation of PPAR-g in T cell uniquely modulate genes, which include metabolic as well genes related to immune function.
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| Overall design |
We used in vitro system to obtain gene expression data. In vitro stimulated naive CD8+ T cells were treated with each PPAR isoforms agonist separately for 48 hours.
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| Contributor(s) |
Chowdhury PS, Kumar A, Chamoto K, Honjo T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 05, 2020 |
| Last update date |
Dec 08, 2020 |
| Contact name |
partha sarathi chowdhury |
| E-mail(s) |
org.partha@gmail.com
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| Organization name |
kyoto university
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| Street address |
Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan
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| City |
kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA682746 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162721_AF_9552_RMA_Value.xlsx |
10.9 Mb |
(ftp)(http) |
XLSX |
| GSE162721_RAW.tar |
10.4 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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