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| Status |
Public on Jan 20, 2010 |
| Title |
Genes regulated by miR-181c |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We found frequently down-regulation of microRNA-181c in human gastric cancer cases. To identify the potential target of miR-181c, we introduced miR-181c into a gastric cancer cell line KATO-III　and analyzed significant changes of gene expression which was induced after introducing miR-181c.
Keywords: dose response
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| Overall design |
KATO-III cells were transfected with Precursor Molecule mimicking miR-181c (Ambion) or mock. Precursor Molecules were gave a final concentration of 100 nmol/L by using MicroPorator MP-100 (Digital BioTechnology, Seoul, Korea), according to the manufacturer’s instructions. At 24 h after transfection, cells were harvested for cDNA microarray analysis.
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| Contributor(s) |
Hashimoto Y, Akiyama Y, Otsubo T, Shimada S, Yuasa Y |
| Citation(s) |
20080834 |
| Submission date |
May 27, 2009 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Yutaka Hashimoto |
| E-mail(s) |
yhashi.monc@live.jp
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| Organization name |
Tokyo Medical and Dental University Graduate School
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| Department |
Molecular Oncology
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| Lab |
Yasuhito Yuasa
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| Street address |
1-5-45
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| City |
Yushima, Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8510 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA115123 |
