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| Status |
Public on Feb 27, 2023 |
| Title |
GEMC1 and MCIDAS interactions with SWI/SNF complexes regulate the multiciliated cell-specific transcriptional program |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Multiciliated cells (MCCs) project dozens to hundreds of motile cilia from their apical surface to promote the movement of fluids or gametes in the mammalian brain, airway or reproductive organs. Differentiation of MCCs requires the sequential action of the Geminin family transcriptional activators, GEMC1 and MCIDAS, that both interact with E2F4/5-DP1. How these factors activate transcription and the extent to which they play redundant functions remains poorly understood. Here, we carried out a transcriptomic analysis to compare the relative influence of GEMC1 and MCIDAS on gene expression.
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| Overall design |
HEK293T cells were transiently transfected with control FLAG vector, or FLAG-tagged GEMC1 or MCIDAS. Cells were harvested in Trizol 48 hours post-transfection for RNA purification and microarray analysis.
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| Contributor(s) |
Stracker T, Lewis M, Terre B, Reina O |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 10, 2020 |
| Last update date |
Mar 01, 2023 |
| Contact name |
Oscar Reina Garcia |
| E-mail(s) |
oscar.reina@irbbarcelona.org
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| Organization name |
IRB Barcelona
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| Department |
Biostatistics and Bioinformatics
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| Street address |
C/Baldiri Reixac 10
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| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA675857 |
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