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| Status |
Public on Aug 01, 2009 |
| Title |
Expression profiling in the Drosophila eye reveals unexpected repression of Notch signaling by the JAK/STAT pathway |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Although the JAK/STAT pathway regulates numerous processes in vertebrates and invertebrates through modulating transcription, its functionally-relevant transcriptional targets remain largely unknown. With one jak and one stat (stat92E), Drosophila provides a powerful system for finding new JAK/STAT target genes. Genome-wide expression profiling on eye discs in which Stat92E is hyperactivated, revealed 584 differentially-regulated genes, including known targets domeless, socs36E and wingless. Other differentially-regulated genes (chinmo, lama, Mo25, Imp-L2, Serrate, Delta) were validated and may represent new Stat92E targets. Genetic experiments revealed that Stat92E cell-autonomously represses Serrate, which encodes a Notch ligand. Loss of Stat92E led to de-repression of Serrate in the dorsal eye, resulting in ectopic Notch signaling and aberrant eye growth there. Thus, our micro-array documents a new Stat92E target gene and a previously-unidentified inhibitory action of Stat92E on Notch signaling. These data suggest that this study will be a useful resource for the identification of additional Stat92E targets.
Identification of the JAK/STAT pathway target genes in the Drosophila eye disc
Keywords: Genotype comparison
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| Overall design |
Gene expression profiles from five biological replicates of eye discs with yw (control) and GMR-upd (overexpressing JAK/STAT ligand unpaired) were compared using genome wide mRNA expression profiling by Affymetrix genechip arrays (Drosophila 2.0) and key targets were validated by clonal analysis, in situ hybridization, immunohistochemical staining and quantitative real-time PCR.
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| Contributor(s) |
Flaherty MS, Zavadil J, Ekas LA, Bach EA |
| Citation(s) |
19504457 |
| Submission date |
Apr 28, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jiri ZAVADIL |
| E-mail(s) |
zavadilj@iarc.fr
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| Phone |
+33648065733
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| Organization name |
IARC WHO
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| Street address |
150 COURS ALBERT THOMAS
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| City |
LYON |
| State/province |
Other |
| ZIP/Postal code |
69008 |
| Country |
France |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (10)
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| GSM398332 |
Mid-third_instar_larval_eye_disc_yw_rep1 |
| GSM398333 |
Mid-third_instar_larval_eye_disc_yw_rep2 |
| GSM398334 |
Mid-third_instar_larval_eye_disc_yw_rep3 |
| GSM398335 |
Mid-third_instar_larval_eye_disc_yw_rep4 |
| GSM398336 |
Mid-third_instar_larval_eye_disc_yw_rep5 |
| GSM398337 |
Mid-third_instar_larval_eye_disc_GMR-upd_rep1 |
| GSM398338 |
Mid-third_instar_larval_eye_disc_GMR-upd_rep2 |
| GSM398339 |
Mid-third_instar_larval_eye_disc_GMR-upd_rep3 |
| GSM398340 |
Mid-third_instar_larval_eye_disc_GMR-upd_rep4 |
| GSM398341 |
Mid-third_instar_larval_eye_disc_GMR-upd_rep5 |
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| Relations |
| BioProject |
PRJNA116973 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15868_RAW.tar |
29.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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