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| Status |
Public on Nov 26, 2020 |
| Title |
Lung Megakaryocytes are Immune Modulatory Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our data demonstrate that lung and bone marrow-derived megakaryocytes (Mks) have distinct immune phenotypes and functions; lung Mks secrete inflammatory cytokines and express molecules that are similar to many tissue resident leukocytes and antigen presenting cells (APCs), and lung Mks process live intact bacteria and present bacteria-derived antigen to CD4+ T cells both in vitro and in vivo. Our in vivo data suggest that lung Mks have important roles in the early activation of T cell responses to a pulmonary pathogen challenge, identifying a novel immune regulatory role for lung Mks.
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| Overall design |
Cells were isolated from either the lung or the bone marrow, and enriched for the megakaryocyte population. Single cell RNA-seq was subsequently performed on isolated cells.
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| Contributor(s) |
Pariser DN, Hilt ZT, Ture SK, Blick-Nitko SK, Looney MR, Cleary SJ, Roman-Pagan E, Saunders J, Georas SN, Veazey J, Madera F, Santos LT, Arne A, Huynh NT, Livada AC, Guerrero-Martin SM, Lyons C, Metcalf-Pate KA, McGrath KE, Palis J, Morrell CN |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 22, 2020 |
| Last update date |
Nov 28, 2020 |
| Contact name |
Farshid Guilak |
| Organization name |
Washington University in St Louis
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| Department |
Orthopaedics
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| Street address |
Couch Building, 3rd Floor, #3213
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| City |
St Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA664993 |
| SRA |
SRP284908 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE158358_RAW.tar |
53.6 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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