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| Status |
Public on Dec 23, 2020 |
| Title |
High-Throughput Analysis of Lung Immune Cells in a Combined Murine Model of Agriculture Dust-Triggered Airway Inflammation with Rheumatoid Arthritis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report identification of 14 unique immune cell populations among different treatment groups with single-cell RNA sequencing high throughput technology. We identified (a) 3 unique neutrophil populations including inflammatory, transient and immunosuppressive/autoreactive granulocytes among experimental groups, (b) heterogeneity among 5 macrophage populations including metabolically active, proliferative, differentiating, recruited, and residential with classical (M1) and alternatively (M2)-activated genes, (c) identification of 2 stages of T-lymphocytes (differentiating and effector), a B-cell population and a NK cell cluster, (d) variability in the distribution of cellular clusters among the treatment groups representing RA and RA-associated lung disease (CIA and CIA+ODE groups, respectively), (e) identification of gMDSC and mMDSC populations based on cell surface markers, and (f) identification of unique genes (interferon-related/autoimmune and complement cascade) that are found in a mouse model of RA-associated inflammatory lung disease. This study identified unique populations of lung immune cell clusters differentially ascribed to individual treatment conditions. We identified neutrophil subpopulations and heterogeneous macrophage-monocyte populations in addition to unique genes (interferon-related and complement cascade) that could be contributing to the pathogenesis of RA-associated lung disease. Additionally, this information might inform potential candidates that could be exploited in future investigations examining targeted interventions and the identification of informative disease biomarkers.
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| |
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| Overall design |
Examination of lung immune cells among treatment groups
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| Contributor(s) |
Gaurav R, Poole JA |
| Citation(s) |
33577605 |
| Submission date |
Jul 30, 2020 |
| Last update date |
Mar 25, 2021 |
| Contact name |
Rohit Gaurav |
| Organization name |
University of Nebraska Medical Center
|
| Department |
Internal Medicine-Allergy
|
| Street address |
985910 Nebraska Medical Center
|
| City |
Omaha |
| State/province |
NE |
| ZIP/Postal code |
68198-5910 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA649761 |
| SRA |
SRP274340 |
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