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| Status |
Public on May 28, 2020 |
| Title |
Gene expression of hypertrophic scar fibroblasts treated with glucocorticoids under mechanical stretch environment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
After disruption of the skin, fibroblasts along wound edges are constantly exposed to high levels of stress, resulting in increased collagen synthesis and decreased apoptosis. Emerge evidences shows that, the hypertrophic scars tissue fibrosis phenomena are original from the intrinsic cellular mechanical stretch.
Traditional glucocorticoids on hypertrophic scarring have been performed for 30 years, utilizing for their anti-inflammatory, anti-allergenic and immunomodulatory effects and for their well-established, pro-apoptotic effects on hematological malignancies. To explore effects of glucocorticoid triamcinolone acetonide for responses to mechanical stress in hypertrophic scars fibroblasts (HSFB), we performed microarrays to explore the differential gene expression.
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| Overall design |
Our protocol included hypertrophic scars fibroblasts treated with glucocorticoid were seeded in the chambers and subjected to mechanical stretch. Paired HSFBs in the control groups were treated with solvent and subjected to mechanical stretch. Then we analyzed using human gene expression arrays.
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| Contributor(s) |
Hu D, Li J, Zhu H |
| Citation(s) |
33627425 |
| Submission date |
May 27, 2020 |
| Last update date |
Mar 16, 2021 |
| Contact name |
Huayu Zhu |
| E-mail(s) |
huayuzhu@fmmu.edu.cn
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| Organization name |
Fourth Military Medical University
|
| Department |
Department of Burns and Cutaneous Surgery, Xijing Hospital
|
| Street address |
Changle Xilu 15
|
| City |
Xi’an |
| State/province |
Shaanxi |
| ZIP/Postal code |
710032 |
| Country |
China |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA635272 |
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