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| Status |
Public on May 19, 2020 |
| Title |
Comparison of Transcriptomes of Wild Type and Paraquat-treated cells by Next Generation RNA Sequencing |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We use RNAseq to compare the transcriptomes of wild type and cells treated with 2.5 mM paraquat (PQ) for 8 hr. We find that PQ treatment results in changes in the transcripts for hundreds of genes. A notable subset of PQ-induced transcripts encodes proteins activated by the retrograde response pathway. This pathway is activated by ETC dysfunction and regulates carbohydrate metabolism to increase synthesis of biosynthetic intermediates through pathways including the TCA cycle, glutamine biosynthetic pathway, and isocitrate metabolism. Transcripts encoding oxidative stress response proteins (e.g. amino acid and iron transporters and siderophores) are also more abundant in PQ-treated cells.
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| Overall design |
mRNA profiles of mid-log wild type (WT) yeast cells and cells with 2.5 mM PQ treatment were generated by deep sequencing
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| Contributor(s) |
Liao P, Pon LA |
| Citation(s) |
32668258 |
| Submission date |
May 18, 2020 |
| Last update date |
Aug 18, 2020 |
| Contact name |
Liza A. Pon |
| E-mail(s) |
lap5@cumc.columbia.edu
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| Organization name |
Columbia University
|
| Department |
Pathology and Cell Biology
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| Street address |
630 W. 168th St., P&S14-442
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL21656 |
Illumina HiSeq 4000 (Saccharomyces cerevisiae) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA633620 |
| SRA |
SRP262215 |
