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| Status |
Public on Jul 21, 2021 |
| Title |
Partial depletion of SRSF1 rescued the C9ORF72-repeat neurodegeneration-associated locomotor deficits in G4C2x36 Drosophila lines |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
In this study we investigated the role of SRSF1 in mitigating locomotor defect in C9ORF72-ALS/FTD Drosophila lines. We analysed the transcriptomes of Drosophila heads from the same lines: (i) control flies expressing 3 G4C2 repeats and a luciferase-RNAi control (G4C2x3 + C-RNAi); (ii) C9ORF72-ALS/FTD model expressing 36 G4C2 repeats and the RNAi control (G4C2x36 + C-RNAi); (iii) C9ORF72-ALS/FTD-neuroprotected flies expressing 36 G4C2 repeats and the disrupted SRSF1 allele (G4C2x36 + ΔSRSF1).
RNA samples were profiled using Drosophila 3' IVT gene expression Affymetrix microarrays prior to qRT-PCR validation of the SRSF1 depletion in neuroprotected flies. This study is part of a larger study that investigates the mitigating role of SRSF1 at whole-genome level to detect transcriptomal alterations and neuronal hyperexcitability in C9ORF72-linked amyotrophic lateral sclerosis.
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| Overall design |
We used RNA from Drosophila heads belonging to the same lines. The over we analysed 9 Affymetrix GeneChip. The experimental design consisted in: (i) 3 samples from control flies expressing 3 G4C2 repeats and a luciferase-RNAi control (G4C2x3 + C-RNAi); (ii) 3 samples C9ORF72-ALS/FTD model expressing 36 G4C2 repeats and the RNAi control (G4C2x36 + C-RNAi); (iii) 3 samples C9ORF72-ALS/FTD-neuroprotected flies expressing 36 G4C2 repeats and the disrupted SRSF1 allele (G4C2x36 + ΔSRSF1).
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| Contributor(s) |
Hautbergue GM, Milo M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 08, 2019 |
| Last update date |
Jul 23, 2021 |
| Contact name |
Marta Milo |
| E-mail(s) |
m.milo@sheffield.ac.uk
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| Organization name |
University of Sheffield
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| Department |
Biomedical Science
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| Street address |
Western Bank
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| City |
Sheffield |
| State/province |
South Yorkshire |
| ZIP/Postal code |
S10 2TN |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (9)
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| GSM4114001 |
Control fly expressing 3 G4C2 repeats and a luciferase-RNAi control. Biological replicate 1 |
| GSM4114002 |
Control fly expressing 3 G4C2 repeats and a luciferase-RNAi control. Biological replicate 2 |
| GSM4114003 |
Control fly expressing 3 G4C2 repeats and a luciferase-RNAi control. Biological replicate 3 |
| GSM4114004 |
C9ORF72-ALS/FTD fly expressing 36 G4C2 repeats and the RNAi control. Biological replicate 1 |
| GSM4114005 |
C9ORF72-ALS/FTD fly expressing 36 G4C2 repeats and the RNAi control. Biological replicate 2 |
| GSM4114006 |
C9ORF72-ALS/FTD fly expressing 36 G4C2 repeats and the RNAi control. Biological replicate 3 |
| GSM4114007 |
C9ORF72-ALS/FTD-neuroprotected fly expressing 36 G4C2 repeats and the disrupted SRSF1 allele. Biological replicate 1 |
| GSM4114008 |
C9ORF72-ALS/FTD-neuroprotected fly expressing 36 G4C2 repeats and the disrupted SRSF1 allele. Biological replicate 2 |
| GSM4114009 |
C9ORF72-ALS/FTD-neuroprotected fly expressing 36 G4C2 repeats and the disrupted SRSF1 allele. Biological replicate 3 |
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| Relations |
| BioProject |
PRJNA576478 |
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