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| Status |
Public on Aug 30, 2019 |
| Title |
Expression data from ex vivo and cultured adipose-derived mesenchymal stromal cells (AdMSC) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The effect of standard tissue culture on AdMSC is not well defined, therefore we sought to analyse global gene changes when a defined ex vivo population of AdMSC was subjected to standard tissue culture conditions.
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| Overall design |
A cocktail of antibodies and immunomagnetic beads were used to enrich for untouched ex vivo AdMSC from the stromal vascular fraction of adipose tissue. We used microarrays to detail the global changes in gene expression when these cells are freshly sorted and compared to when these cells are grown in standard tissue culture conditions for different time periods
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| Contributor(s) |
Sheppard HM |
| Citation(s) |
32153389 |
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Title - Ex Vivo Human Adipose Tissue Derived Mesenchymal Stromal Cells(ASC) Are a Heterogeneous Population That Demonstrate Rapid Culture-Induced Changes Authors - Anna E. S. Brooks, Megan Iminitoff , Eloise Williams, Tanvi Damani, Victoria Jackson-Patel, Vicky Fan, Joanna James, P. Rod Dunbar, Vaughan Feisst and Hilary Sheppard Journal - Frontiers in Pharmacology, vol. 10, article 1695.
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| Submission date |
Aug 29, 2019 |
| Last update date |
Mar 16, 2020 |
| Contact name |
Hilary Sheppard |
| E-mail(s) |
h.sheppard@auckland.ac.nz
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| Organization name |
Auckland University
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| Street address |
Symonds St
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| City |
Auckland |
| ZIP/Postal code |
1050 |
| Country |
New Zealand |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (15)
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| GSM4053594 |
Freshly sorted cells, biological rep 1 |
| GSM4053595 |
Sorted cells cultured for 3 days, biological rep 1 |
| GSM4053596 |
Sorted cells cultured for 28 days, biological rep 1 |
| GSM4053597 |
Sorted cells cultured in adipo medium for 14 days, biological rep 1 |
| GSM4053598 |
Freshly sorted cells, biological rep 2 |
| GSM4053599 |
Sorted cells cultured for 3 days, biological rep 2 |
| GSM4053600 |
Sorted cells cultured for 28 days, biological rep 2 |
| GSM4053601 |
Sorted cells cultured in adipo medium for 14 days, biological rep 2 |
| GSM4053602 |
Freshly sorted cells, biological rep 3 |
| GSM4053603 |
Sorted cells cultured for 3 days, biological rep 3 |
| GSM4053604 |
Sorted cells cultured for 28 days, biological rep 3 |
| GSM4053605 |
Sorted cells cultured in adipo medium for 14 days, biological rep 3 |
| GSM4053606 |
Sorted cells cultured for 9 passages, biological rep 3 |
| GSM4053607 |
Sorted cells cultured for 9 passages, biological rep 1 |
| GSM4053608 |
Sorted cells cultured for 9 passages, biological rep 2 |
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| Relations |
| BioProject |
PRJNA562899 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE136633_RAW.tar |
29.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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