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| Status |
Public on Sep 24, 2019 |
| Title |
Single cell RNA-seq Analysis of Wild Type(WT), Dux knockout(KO) and Dux overexpression(OE) embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We tested the transcriptome of embryos from WT and Dux KO mice including zygotes (hCG 28h), early 2-cell (hCG 31-32h), middle 2-cell(hCG 41-42h), late 2-cell(hCG 47-48h) and, Dux mRNA injected embryos including zygote(5h after injection, hCG 28h), early 4-cell(6h after injection, hCG 54h) and late 4-cell (17h after injection, hCG 65h) using the Covaris DNA shearing protocol for Smart-seq sequence library generation. We found that the activation of Dux is important but not essential for ZGA, but the silencing and elimination of Dux is strictly necessary for early embryonic development.
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| Overall design |
Embryos mRNA profiles of WT,Dux KO and Dux OE were generated by deep sequencing, using Illumina NovaSeq 6000.
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| Contributor(s) |
Guo M, Zhang Y, Wang Y |
| Citation(s) |
31591446 |
| Submission date |
May 31, 2019 |
| Last update date |
Oct 30, 2019 |
| Contact name |
Mingyue Guo |
| E-mail(s) |
1610815@tongji.edu.cn
|
| Phone |
86-21-65982278
|
| Organization name |
Tongji University
|
| Department |
School of Life science and technology
|
| Lab |
Gao Shaorong
|
| Street address |
Siping
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (99)
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| This SubSeries is part of SuperSeries: |
| GSE134832 |
Precise temporal regulation of Dux is important for embryo development |
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| Relations |
| BioProject |
PRJNA548396 |
| SRA |
SRP201141 |
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