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| Status |
Public on Apr 16, 2020 |
| Title |
The Ccr4-Not complex monitors the translating ribosome for codon optimality |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
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| Summary |
Control of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA decay through deadenylation and activation of decapping. We used a combination of cryo–electron microscopy, ribosome profiling, and mRNA stability assays to examine the recruitment of Ccr4-Not to the ribosome via specific interaction of the Not5 subunit with the ribosomal E-site in Saccharomyces cerevisiae. This interaction occurred when the ribosome lacked accommodated A-site transfer RNA, indicative of low codon optimality. Loss of the interaction resulted in the inability of the mRNA degradation machinery to sense codon optimality. Our findings elucidate a physical link between the Ccr4-Not complex and the ribosome and provide mechanistic insight into the coupling of decoding efficiency with mRNA stability.
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| Overall design |
2 replicates in 2 different strains
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| Contributor(s) |
Buschauer R, Matsuo Y, Sugiyama T, Chen Y, Alhusaini N, Sweet T, Ikeuchi K, Cheng J, Matsuki Y, Nobuta R, Gilmozzi A, Berninghausen O, Tesina P, Becker T, Coller J, Inada T, Beckmann R |
| Citation(s) |
32299921 |
| Submission date |
May 17, 2019 |
| Last update date |
Jun 10, 2020 |
| Contact name |
Yoshitaka Matsuo |
| Organization name |
Tohoku university
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| Street address |
Aoba 6-3, Aoba-Ku
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| City |
Sendai |
| ZIP/Postal code |
980-8578 |
| Country |
Japan |
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| Platforms (1) |
| GPL21656 |
Illumina HiSeq 4000 (Saccharomyces cerevisiae) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA543493 |
| SRA |
SRP198768 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE131408_RAW.tar |
77.5 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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