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| Status |
Public on Jul 29, 2019 |
| Title |
Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing [WGBS] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We systematically evaluated alternate strategies for WGBS studies, taking into account opportunites around library preparation methods, sequencing platforms and analysis pipeline optimization. We also assessed the performance and precision of the WGBS method relative to the methylation arrays, in an effort to provide data-driven recommendations for future WGBS studies, in particularly with respect to minimum coverage.
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| Overall design |
Bisulfite-converted DNA for 8 human samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip and Illumina Infinium HumanMethylation850 BeadChip. Whole genome bisulphite sequencing libraries for 8 human samples were prepared by different methods with low DNA input requirement and sequenced by Illumina NovaSeq and HiSeq X10 platforms.
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| Contributor(s) |
Zhou L, Ng HK, Moses D, Schuster SC, Beck S, Kim C, Chambers JC, Loh M |
| Citation(s) |
31316107 |
| Submission date |
Mar 22, 2019 |
| Last update date |
Aug 04, 2019 |
| Contact name |
LI ZHOU |
| E-mail(s) |
zhou.li@ntu.edu.sg
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| Organization name |
Lee Kong Chian School of Medicine, Nanyang Technological University
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| Street address |
11 Mandalay Road
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| City |
Singapore |
| ZIP/Postal code |
308232 |
| Country |
Singapore |
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| Platforms (2) |
| GPL20795 |
HiSeq X Ten (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (41)
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| This SubSeries is part of SuperSeries: |
| GSE128734 |
Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing |
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| Relations |
| BioProject |
PRJNA528657 |
| SRA |
SRP217135 |
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