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| Status |
Public on Jul 29, 2019 |
| Title |
Anti-inflammatory nutrition with high protein attenuates cardiac and skeletal muscle alterations in a pulmonary arterial hypertension model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Pulmonary arterial hypertension (PAH) is characterized by remodelling of the pulmonary arteries and right ventricle (RV), which leads to functional decline of cardiac and skeletal muscle. This study investigated the effects of a multi-targeted nutritional intervention with extra protein, leucine, fish oil and oligosaccharides on cardiac and skeletal muscle in PAH. PAH was induced in female C57BL/6 mice by weekly injections of monocrotaline (MCT) for 8 weeks. Control diet (sham and MCT group) and isocaloric nutritional intervention (MCT + NI) were administered. Compared to sham, MCT mice increased heart weight by 7%, RV thickness by 13% and fibrosis by 60% (all p < 0.05) and these were attenuated in MCT + NI mice. Microarray and qRT-PCR analysis of RV confirmed effects on fibrotic pathways. Skeletal muscle fiber atrophy was induced (P < 0.05) by 22% in MCT compared to sham mice, but prevented in MCT + NI group. Our findings show that a multi-targeted nutritional intervention attenuated detrimental alterations to both cardiac and skeletal muscle in a mouse model of PAH, which provides directions for future therapeutic strategies targeting functional decline of both tissues.
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| Overall design |
Microarray analysis was performed on right ventricle biopsies from mice that received injections with either monocrotaline (MCT) or saline (sham) and were fed a standard semi-synthetic diet or isocaloric nutritional intervention (NI).
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| Contributor(s) |
Vinke P, Bowen TS, Boekschoten MV, Witkamp RF, Adams V, van Norren K |
| Citation(s) |
31308383 |
| Submission date |
Jan 23, 2019 |
| Last update date |
Aug 11, 2019 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
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| Organization name |
Wageningen University
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| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM3576738 |
Right ventricle, MCT, mouse 640 |
| GSM3576739 |
Right ventricle, MCT, mouse 641 |
| GSM3576740 |
Right ventricle, MCT, mouse 644 |
| GSM3576741 |
Right ventricle, MCT+NI, mouse 615 |
| GSM3576742 |
Right ventricle, MCT+NI, mouse 617 |
| GSM3576743 |
Right ventricle, MCT+NI, mouse 619 |
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| Relations |
| BioProject |
PRJNA516702 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125537_RAW.tar |
39.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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