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| Status |
Public on Jan 01, 2020 |
| Title |
Tri-cultures of HGSOC cells replicate tissue remodelling observed in biopsies. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We built novel tri-cultures of HGSOC cells with human primary omental adipocytes and fibroblasts which replicated tissue remodelling and matrisome production observed in biopsies. RNAseq analysis showed that tri-cultures reproduced the prognostic matrisome signature.
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| Overall design |
RNA sequencing on 1) 3D-tricultures of HGSOC malignant cells, omental fibroblasts and adipocytes with (n = 2) or without (n = 2) TGFβRI + GLI1/2 inhibitors, or on adipocyte-only gels (n = 1), or co-cultures of HGSOC malignant cells and omental fibroblasts (n = 2); and 2) on two HGSOC malignant cell lines grown in monolayer (n = 2-3) and as spheroids (n = 2-3).
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| |
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| Contributor(s) |
Delaine-Smith R, Maniati E, Malacrida B, Balkwill F |
| Citation(s) |
34189438 |
| Submission date |
Jan 15, 2019 |
| Last update date |
Jul 08, 2021 |
| Contact name |
Eleni Maniati |
| E-mail(s) |
e.maniati@qmul.ac.uk
|
| Organization name |
Queen Mary University of London
|
| Department |
Barts Cancer Institute
|
| Lab |
Cancer and Inflammation
|
| Street address |
John Vane Science Centre, Charterhouse Square
|
| City |
London |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
| |
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| Platforms (2) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA515261 |
| SRA |
SRP179510 |
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