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Series GSE12424 Query DataSets for GSE12424
Status Public on Aug 28, 2015
Title Renal Expression of Dahl SS/Jr (S) and Congenic Rats after 24 hours of Dietary Salt Loading
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The overlap of congenic regions in an earlier substitution mapping study suggested the location of two blood pressure quantitative trait loci (QTL)-containing regions in the q-terminus of rat chromosome 3, QTL1 and the more distal, QTL2. Male SS/jr rats and two congenic substrain rats S.R(D3Mco36-D3Mco46) and S.R(D3Mco36-D3Got166) were maintained on a low salt (0.4% NaCl Harlan Teklad diet TD7034) diet until 39-41 days of age. These 3 rat strains will be hereafter referred to as S, S.R(ET3x1), and S.R(ET3x2), respectively. At 39-41 days of age, half of the rats from each strain were fed a high (4% NaCl Harlan Teklad diet TD83033) salt diet and water ad libitum for 24 hours. Both congenic substrains carry SR/jr (R)-rat alleles for QTL2 on an S-rat genetic background, while S.R(ET3x2) also carries R-rat alleles for QTL1. Renal gene expression analysis was used to identify differentially expressed genes or genes with altered activity within the S.R(ET3x2) congenic region.

Keywords: strain differences in response to dietary changes
 
Overall design Age-matched male S (n=10), S.R(ET3x1) (n=8), and S.R(ET3x2) (n=8) rats were bred, housed, and studied concomitantly to minimize envi¬ronmental effects. Rats were weaned at 30 days of age onto a low salt diet (0.4% NaCl, Harlan Teklad diet TD7034) and water ad libitum. At 39-41 days of age one-half of rats from each strain group were switched to a 4% NaCl diet (Harlan Teklad diet TD83033) and water ad libitum for 24 hours. Rats were then killed with pentobarbitol overdose. Gene expression data from Affymetrix Rat RAE230 A and B GeneChips, hybridized with kidney cRNA, was used to identify genes with altered activity within the S.R(ET3x2) congenic region.
 
Contributor(s) McSweeny AJ, Jin Lee S, Cicila GT
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Submission date Aug 12, 2008
Last update date Mar 03, 2017
Contact name Andrew John McSweeny
E-mail(s) andrew.mcsweeny@utoledo.edu
Phone +1 419 383 4182
Fax +1 419 383 2871
URL http://hsc.utoledo.edu/grad/cvmd/faculty.html
Organization name University of Toledo College of Medicine
Department Physiology & Pharmacology: CVMD Track
Lab George T. Cicila
Street address 3000 Arlington Avenue Room # 209, Mail Stop #1008
City Toledo
State/province OH
ZIP/Postal code 43614
Country USA
 
Platforms (2)
GPL341 [RAE230A] Affymetrix Rat Expression 230A Array
GPL342 [RAE230B] Affymetrix Rat Expression 230B Array
Samples (52)
GSM312064 S_rat_high_salt_diet_S7416_kidney_RNA_RAE230A
GSM312065 S_rat_high_salt_diet_S7417_kidney_RNA_RAE230A
GSM312066 S_rat_high_salt_diet_S7418_kidney_RNA_RAE230A
This SubSeries is part of SuperSeries:
GSE29078 Dietary Salt effects on Renal Expression of Dahl SS/Jr (S) and Congenic Rats
Relations
BioProject PRJNA141673

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12424_RAW.tar 128.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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