|
| Status |
Public on Oct 15, 2018 |
| Title |
Targeting individual cells by barcode in pooled sequence libraries |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Selectively sequence desired single cells from pooled single cell libraries
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| |
|
| Overall design |
10X libraries were prepared from two FACs sorted populations (HLA-DR and CD19) followed by enrichment for targeted single cells in triplicate. Enrichment was achieved with multiplex PCR with primers targeting the single cell barcodes that are added through library preparation.
NOTE: Submitter states that the raw data will be submitted to dbGaP (https://www.ncbi.nlm.nih.gov/gap) because of patient privacy concerns.
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| |
|
| Contributor(s) |
Ranu N |
| Citation(s) |
30256981 |
| Submission date |
Jul 05, 2018 |
| Last update date |
Mar 20, 2019 |
| Contact name |
Navpreet Ranu |
| E-mail(s) |
nranu@broadinstitute.org
|
| Organization name |
Broad Institute
|
| Street address |
415 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (2) |
|
| Samples (4)
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|
| Relations |
| BioProject |
PRJNA479863 |
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