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| Status |
Public on Nov 20, 2018 |
| Title |
Transcriptome Profiling of Japanese Encephalitis Virus (JEV) Infected Human Neuroepithelioma Cells (SK-N-MC) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Japanese Encephalitis (JE) is an important causal of viral encephalitis in Eastern and Southeast Asia. Neuronal injury and varying degrees of neuroinflammation are often pathological hallmarks of JE, which result in high morbility and mortality rates. However, a clear understanding on neuron cells' responses to JEV infection remains elusive. This study, therefore, utilized the Affymetrix Human PrimeView array for a robust profiling of JEV infected SK-N-MC cells during the active phase of infection in order to investigate the molecular details of the host cell responses.
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| Overall design |
SK-N-MC cells were mock and JEV infected at Multiplicity of Infection (MOI) 10. Mock infections were achieved by subtitution of the viruses with equal amount of medium. Total RNA of mock and JEV infected cells were extracted at 48 and 60 hour post infection. Total RNA to be hybridized to Affymetrix Human PrimeView arrays were processed using the Affymetrix GeneChip 3' IVT Plus kits. Three biological replicates were used for both time points post infection.
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| Contributor(s) |
Yu SP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 31, 2018 |
| Last update date |
Nov 21, 2018 |
| Contact name |
Shu Pin Yu |
| E-mail(s) |
yushupin@gmail.com
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| Organization name |
University Malaya
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| Department |
Pathology
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| Lab |
Developmental Lab 2
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| Street address |
Kuala Lumpur
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| City |
kuala lumpur |
| State/province |
kuala lumpur |
| ZIP/Postal code |
50603 |
| Country |
Malaysia |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (12)
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| GSM3168451 |
no infection, 48 hour post infection, biological replicate 1 |
| GSM3168452 |
no infection, 48 hour post infection, biological replicate 2 |
| GSM3168453 |
no infection, 48 hour post infection, biological replicate 3 |
| GSM3168454 |
JEV infected, 48 hour post infection, biological replicate 1 |
| GSM3168455 |
JEV infected, 48 hour post infection, biological replicate 2 |
| GSM3168456 |
JEV infected, 48 hour post infection, biological replicate 3 |
| GSM3168457 |
no infection, 60 hour post infection, biological replicate 1 |
| GSM3168458 |
no infection, 60 hour post infection, biological replicate 2 |
| GSM3168459 |
no infection, 60 hour post infection, biological replicate 3 |
| GSM3168460 |
JEV infected, 60 hour post infection, biological replicate 1 |
| GSM3168461 |
JEV infected, 60 hour post infection, biological replicate 2 |
| GSM3168462 |
JEV infected, 60 hour post infection, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA473994 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE115167_RAW.tar |
27.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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