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Status |
Public on Sep 10, 2020 |
Title |
Next Generation Sequencing Analysis of Human Glioblastoma Transcriptomes And Quantitative Analysis of PDGFRA Positive/Negative Human Glioma Cells Transcriptomes |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We report the high-throughput profiling of thirteen human glioblastoma and four pairs of panned(pdgfra+) and supernatant(pdgfra-) human glioma cells. The subtypes and the different expression genes(DEGs) was identified integrated genomic analysis:single sample GSEA and DESeq.We obtained the enrichment gene pathway by using Gene Ontology Enrichment analysis-Functional classification viewed in pie chart.We find that the features of Proneural were enriched generaly when comparing the four pairs of the panned(pdgfra+) and supernatant(pdgfra-) human glioma cells.
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Overall design |
Seven Glioblastoma tissue and four pairs of panned(pdgfra+) and supernatant(pdgfra-) human glioma cells were generated by deep sequencing, using Illumina Hiseq X_Ten.
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Contributor(s) |
liu C |
Citation(s) |
33173731 |
Submission date |
Jan 08, 2018 |
Last update date |
Dec 10, 2020 |
Contact name |
Chong Liu |
E-mail(s) |
0015027@zju.edu.cn
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Organization name |
Medical College of Zhejiang University
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Street address |
NO.866 of Yuhang Tong Road
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City |
Zhejiang |
ZIP/Postal code |
310058 |
Country |
China |
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Platforms (1) |
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Samples (15)
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Relations |
BioProject |
PRJNA428971 |
SRA |
SRP130951 |