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| Status |
Public on Nov 07, 2017 |
| Title |
BMAL1-deficiency contributes to mandibular dysplasia by upregulating MMP3 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Skeletal mandibular hypoplasia (SMH), one of the common types of craniofacial deformities, seriously affects appearance, chewing, pronunciation, and breathing. Moreover, SMH is prone to inducing obstructive sleep apnea syndrome. We found that brain and muscle ARNT-like 1 (BMAL1), the core component of the molecular circadian oscillator, was significantly decreased in mandibles of juvenile SMH patients. Accordingly, SMH was observed in circadian rhythm-disrupted or BMAL1-deficiency mice. RNA sequencing and protein chip analyses suggested that matrix metallopeptidase 3 (MMP3) is the potential target of BMAL1 during the mandibular developmental process. Interestingly, in juvenile SMH patients, we observed that MMP3 level was increased obviously. Consistently, MMP3 was upregulated during the whole growth period of 3 to 10 weeks in Bmal1-/- mice. Given these findings, we set to characterize the underlying mechanism and found that BMAL1-deficiency enhanced Mmp3 transcription through activating p65 phosphorylation. Together our results provide new insight into the mechanism by which BMAL1 is implicated in the pathogenesis of SMH
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| Overall design |
To gain an independent assessment of the regulatory targets of BMAL1 in Bmal1-/- mBMSCs
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| Contributor(s) |
Zhao J, Chen L |
| Citation(s) |
29276151 |
| Submission date |
Nov 06, 2017 |
| Last update date |
Jul 10, 2019 |
| Contact name |
Lili Chen |
| E-mail(s) |
tangting-0@qq.com
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| Organization name |
Union Hospital, Tongji Medical College Huazhong University of Science and Technology
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| Street address |
1277 Jiefang Avenue
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430022 |
| Country |
China |
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| Platforms (1) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA417294 |
| SRA |
SRP124290 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106586_RAW.tar |
10.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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