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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 21, 2004 |
| Title |
Myometrial cells expressing CREB, CREM alpha, CREM tau2alpha, ATF2 or the ATF2-small gene |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human myometrial cells taken from biopsies of pre-menopausal non-cancerous non-pregnant uteri were cultured in complete MEM D-valine medium plus 10% FCS, and stably-transfected with the pcDNA3.1/V5-His TOPO vector (Invitrogen) harbouring either CREB, CREM alpha, CREM tau2alpha, ATF2, ATF2-small gene or a control empty-vector. Total RNA was extracted using TRI-reagent (Sigma) and RNeasy columns (Qiagen), and 10 ug used in target preparation for array hybridization.
Keywords: other
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| Contributor(s) |
Bailey J, Europe-Finner GN |
| Citation(s) |
15691874 |
| Submission date |
Feb 20, 2004 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Jarrod Bailey |
| E-mail(s) |
jarrod.bailey@ncl.ac.uk
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| Phone |
(+44) 191 2228748
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| Fax |
(+44) 191 2225066
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| Organization name |
University of Newcastle upon Tyne
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| Department |
School of Surgical and Reproductive Sciences
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| Street address |
Leech Building, Medical School
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| City |
Newcastle upon Tyne |
| State/province |
Tyne and Wear |
| ZIP/Postal code |
NE2 4HH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (18)
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| GSM17039 |
Control human myometrial cells |
| GSM17040 |
Control empty-vector stably-transfected human myometrial cells |
| GSM17041 |
Control empty-vector stably-transfected human myometrial cells (replicate) |
| GSM17042 |
Human myometrial cells stably-transfected with the CREB gene |
| GSM17059 |
Human myometrial cells stably-transfected with the CREB gene, replicate 2 |
| GSM17060 |
Human myometrial cells stably-transfected with the CREB gene, replicate 3 |
| GSM17061 |
Human myometrial cells stably-transfected with the CREM aplha gene |
| GSM17062 |
Human myometrial cells stably-transfected with the CREM aplha gene, replicate 2 |
| GSM17063 |
Human myometrial cells stably-transfected with the CREM aplha gene, replicate 3 |
| GSM17064 |
Human myometrial cells stably-transfected with the CREM tau2alpha gene |
| GSM17065 |
Human myometrial cells stably-transfected with the CREM tau2alpha gene, replicate 2 |
| GSM17066 |
Human myometrial cells stably-transfected with the CREM tau2alpha gene, replicate 3 |
| GSM17067 |
Human myometrial cells stably-transfected with the ATF2 gene |
| GSM17068 |
Human myometrial cells stably-transfected with the ATF2 gene, replicate 2 |
| GSM17069 |
Human myometrial cells stably-transfected with the ATF2 gene, replicate 3 |
| GSM17070 |
Human myometrial cells stably-transfected with the ATF2-small gene |
| GSM17071 |
Human myometrial cells stably-transfected with the ATF2-small gene, replicate 2 |
| GSM17072 |
Human myometrial cells stably-transfected with the ATF2-small gene, replicate 3 |
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| Relations |
| BioProject |
PRJNA87139 |
| Supplementary data files not provided |
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