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| Status |
Public on Jan 15, 2018 |
| Title |
BCR heavy and light chain repertoire sequencing of pertitoneal cavity B-1a cells from wild-type and Igll1 knockout mice |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
We report that B-1a cells develop in a surrogate light chain independent context. As a consequence, the precursor B-1a cell population avoids a pre-BCR positive selection stage. To confirm that the B-1a cells generated in this manner repersent a bonafide B-1a cell compartment, we did NGS on BCR rearrangements to assess the repertoire diversity. We find that as a whoile, B-1a cell repertoire that develop in Igll1 kncokout mice are similar compared to wild-type. This supports our findings that B-1a cells develop properly in the absence of surrogate light chain.
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| Overall design |
Peritoneal cavity B-1a cells were sorted from 6-8 week old mice, DNA was extracted and rearranged heavy chains were amplified by PCR. These PCR products were then process to form DNA libraries that were compatible with Illumina NGS.
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| Contributor(s) |
Skok JA, Wong JB |
| Citation(s) |
31628339 |
| Submission date |
Sep 21, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jane Skok |
| E-mail(s) |
jane.skok@nyumc.org
|
| Organization name |
NYU
|
| Street address |
550 First Ave, Smillow 307
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (2) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA408276 |
| SRA |
SRP118496 |
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