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| Status |
Public on Nov 12, 2017 |
| Title |
Staufen1 inhibits MyoD translation to actively maintain muscle stem cell quiescence |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Tissue regeneration depends on the timely activation of adult stem cells. In skeletal muscle, the adult stem cells maintain a quiescent state and proliferate upon injury. We show that muscle stem cells (MuSCs) use direct translational repression to maintain the quiescent state. High resolution single molecule and single cell analyses demonstrate that quiescent MuSCs express high levels of Myogenic Differentiation1 (MyoD) transcript in vivo, whereas MyoD protein is absent. RNA pulldowns and co-stainings show that MyoD mRNA interacts with Staufen1, a potent regulator of mRNA localization, translation, and stability. Staufen1 prevents MyoD translation through its interaction with the MyoD 3’UTR. MuSCs from Staufen1 heterozygous (Staufen1+/-) mice have increased MyoD protein expression, exit quiescence, and begin proliferating. Conversely, blocking MyoD translation maintains the quiescent phenotype. Collectively, our data show that MuSCs express MyoD mRNA and actively repress its translation to remain quiescent yet primed for activation.
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| Overall design |
Anti-Staufen1 RIP-seq in quiescent MuSCs
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| Contributor(s) |
Rando TA, de Morree A, van Velthoven CT |
| Citation(s) |
29073096 |
| Submission date |
Sep 07, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Cindy van Velthoven |
| E-mail(s) |
cindy.vanvelthoven@alleninstitute.org
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| Phone |
+12065487000
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| Organization name |
Allen Institute
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| Street address |
615 Westlake Ave N
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA402057 |
| SRA |
SRP117056 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE103603_StauRIP_annotated_counts_20170124.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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