Genome binding/occupancy profiling by high throughput sequencing
Summary
We monitored changes to genomic binding of BRG1 andBRM following BRG1 or BRM loss to understand how disuprtion of one member of the SWI/SNF complex affected remaining forms of the complex present in the cell.
Overall design
ChIP-seq was performed for BRG1 and BRM in HepG2 cells treated with non-targeting, BRG1 shRNA , or BRM shRNA
Jesse Raab, John S. Runge, Camarie C. Spear, and Terry Magnuson. Co-regulation of transcription by BRG1 and BRM, two mutually exclusive SWI/SNF ATPase subunits. bioRxiv 178848, 2017. doi:10.1101/178848
NIH grant(s)
Grant ID
Grant title
Affiliation
Name
R01 HD036655
Developmental Gene Regulation Via Chromatin Remodeling