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| Status |
Public on Dec 31, 2018 |
| Title |
Endothelial progenitor cells contribute to neovascularization of non-small cell lung cancer via HDAC7 mediated cytoskeleton regulation and angiogenic genes transcription |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To supply tumor tissues with nutrients and oxygen, endothelial progenitor cells (EPCs) are recruited to tumor bed and contribute to tumor neovascularization. HDAC7 is a critical regulator in EPC-mediated neovascularization.
To explore the function of HDAC7 in neovascularization induced by EPCs, we use microarrays to profile the genes expression changes in HDAC7 lacking EPCs. HDAC7 silencing upregulated antiangiogenic genes transcription and suppressed proangiogenic genes expression, turning off the angiogenic switch for vessel formation.
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| Overall design |
EPCs were isolated from human umbilical cord blood of healthy volunteers and the 3rd passage of cultured EPCs were selected for lentivirus infection. EPCs isolated from the same volunteer were devided into KD and NC group. EPCs were infected with lentivirus carrying HDAC7 shRNA in KD group and nonspecific random shRNA in NC group. Each group contains 2 samples from different volunteers for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Wei Y, Zhou F, Zhou H, Huang J, Yu D, Wu G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 02, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Ye Wei |
| Organization name |
Cancer Center, Union Hospital
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| Street address |
Tongji Medical College, Huazhong University of Science and Technology
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430022 |
| Country |
China |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA396927 |
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