VGlut Vesicular glutamate transporter [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: VGlutprovided by FlyBase
Official Full Name: Vesicular glutamate transporterprovided by FlyBase
Primary source: FLYBASE:FBgn0031424
Locus tag: Dmel_CG9887
See related: AllianceGenome:FB:FBgn0031424
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG9887; Dmel\CG9887; DV-Glut; dvglut; dvGlut; dvGluT; dVGlut; dVGLUT; DvGlut; DvGluT; DVGlut; DVGluT; DVGLUT; MFS11; vglut; vGlut; vGluT; vGLUT; Vglut; VGluT; VGLUT
Summary: Enables neurotransmitter transmembrane transporter activity. Involved in glutamatergic synaptic transmission; positive regulation of neuromuscular synaptic transmission; and regulation of membrane potential in photoreceptor cell. Located in synaptic vesicle. Is expressed in several structures, including adult head; glutamatergic neurons; leg; and nervous system. Used to study Parkinson's disease and neurodegenerative disease. Human ortholog(s) of this gene implicated in autosomal dominant nonsyndromic deafness 25. Orthologous to several human genes including SLC17A7 (solute carrier family 17 member 7) and SLC17A8 (solute carrier family 17 member 8). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 22E1-22E1; 2-6 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (2400295..2410662)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (2391660..2410662)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Vesicular glutamate transporter modulates sex differences in dopamine neuron vulnerability to age-related neurodegeneration.
Title: Vesicular glutamate transporter modulates sex differences in dopamine neuron vulnerability to age-related neurodegeneration.
PICALM rescues glutamatergic neurotransmission, behavioural function and survival in a Drosophila model of Abeta42 toxicity.
Title: PICALM rescues glutamatergic neurotransmission, behavioural function and survival in a Drosophila model of Aβ42 toxicity.
Study established that the majority of Drosophila octopamine (OA) neurons are also glutamatergic and identify the individual contributions of each neurotransmitter on sex-specific behaviors. Males deficient for dVGLUT solely in OA-glutamate neurons also exhibit a reduction in aggression, but without a concurrent increase in inter-male courtship. Study revealed previously undetermined role for dVGLUT in OA neurons.
Title: Octopamine neuron dependent aggression requires dVGLUT from dual-transmitting neurons.
This study demonstrated that Astrocytic glutamate transport regulates a Drosophila CNS synapse that lacks astrocyte ensheathment.
Title: Astrocytic glutamate transport regulates a Drosophila CNS synapse that lacks astrocyte ensheathment.
Overexpression of Drosophila GLUT is sufficient to generate excitotoxic neuropathological phenotypes; therefore, reducing VGLUT levels after nervous system injury or stress may mitigate further damage.
Title: Increased vesicular glutamate transporter expression causes excitotoxic neurodegeneration.
In the larval CNS,synaptic DVGLUT in all brain lobe neuropil compartments except for the mushroom body. Likewise in the adult CNS, glutamatergic synapses are abundant throughout all major brain structures except the mushroom body.
Title: Visualizing glutamatergic cell bodies and synapses in Drosophila larval and adult CNS.
DVGLUT contains 10 hydrophobic regions that completely span the membrane (TMs 1-10) & that the amino & carboxyl termini are cytosolic. between TMs 4 & 5 is an unforeseen cytosolic loop of some 50 residues.
Title: Membrane topology of the Drosophila vesicular glutamate transporter.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables L-glutamate transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables L-glutamate transmembrane transporter activity	IC Inferred by Curator more info	PubMed
enables L-glutamate transmembrane transporter activity	ISA Inferred from Sequence Alignment more info	PubMed
enables neurotransmitter transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables neurotransmitter transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in L-glutamate transmembrane transport	ISA Inferred from Sequence Alignment more info	PubMed
involved_in monoatomic anion transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic cation transport	IDA Inferred from Direct Assay more info	PubMed
involved_in neurotransmitter loading into synaptic vesicle	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of neuromuscular synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cellular pH	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of membrane potential in photoreceptor cell	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of synapse structure or activity	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic transmission, glutamatergic	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic transmission, glutamatergic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in excitatory synapse	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	ISM Inferred from Sequence Model more info	PubMed
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
is_active_in synaptic vesicle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in synaptic vesicle membrane	IC Inferred by Curator more info	PubMed
located_in vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: vesicular glutamate transporter

Names: CG9887-PA; CG9887-PB; VGlut-PA; VGlut-PB; drosophila vesicular glutamate transporter

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

2400295..2410662

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_134837.3 → NP_608681.2 vesicular glutamate transporter, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_608681.2

Status: REVIEWED

UniProtKB/TrEMBL

Q86P76, Q9VQC0

Conserved Domains (2) summary

cd06174
Location:98 → 512

MFS; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of ...

pfam07690
Location:101 → 475

MFS_1; Major Facilitator Superfamily
NM_001258919.1 → NP_001245848.1 vesicular glutamate transporter, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001245848.1

Status: REVIEWED

UniProtKB/TrEMBL

Q86P76, Q9VQC0

Conserved Domains (2) summary

cd06174
Location:98 → 512

MFS; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of ...

pfam07690
Location:101 → 475

MFS_1; Major Facilitator Superfamily