GEO DataSets

(Submitter supplied) Photosynthetic microbes can produce the clean-burning fuel hydrogen using one of nature’s most plentiful resources, sunlight 1,2. Anoxygenic photosynthetic bacteria generate hydrogen and ammonia during a process known as biological nitrogen fixation. This reaction is catalyzed by the enzyme nitrogenase and consumes nitrogen gas, ATP and electrons 3. One bacterium, Rhodopseudomonas palustris, has a remarkable ability to obtain electrons from green plant-derived material 4,5 and to efficiently absorb both high and low intensity light energy to form ATP 6. more...

Organism:

Rhodopseudomonas palustris

Type:

Expression profiling by array

Platform:

GPL3954

14 Samples

Download data: CEL, EXP

(Submitter supplied) Transcriptome analysis was performed in order to better understand the metabolic activity of non-growing cells of Rhodopseudomonas palustris for improve biofuel production.

Organism:

Rhodopseudomonas palustris CGA009

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17852

14 Samples

Download data: TXT

(Submitter supplied) To address the question of how photosynthetic bacterium Rhodopseudomonas palustris differentially regulates gene expression of three nitrogenase isozymes (Mo, V, and Fe nitrogenases), we constructed Mo strain (Mo nitrogenase only strain), V strain (V nitrogenase only strain), and Fe strain (Fe nitrogenase only strain), and analyzed the whole genome transcriptome profiles of each mutant and wild-type cells grown under nitrogen-fixing conditions. more...

Organism:

Rhodopseudomonas palustris

Type:

Expression profiling by array

Platform:

GPL2697

50 Samples

Download data: XLS

(Submitter supplied) Characterization of post-translational modification of nitrogenase in Rhodopseudomonas palustris strains that produce hydrogen gas constitutively.

Organism:

Rhodopseudomonas palustris

Type:

Expression profiling by array

Platforms:

GPL2697 GPL3954

22 Samples

Download data: CEL, TXT

(Submitter supplied) We used transcriptome analysis to identify genes that were differentially expressed between wild-type (CGA010) and hupV mutant cells (CGA009) grown photoheterotrophically with malate under nitrogen-fixing conditions. We also compared the transcriptome of a hupS mutant with that of the wild type. Keywords: Genetic modification

Organism:

Rhodopseudomonas palustris

Type:

Expression profiling by array

Platform:

GPL2697

16 Samples

Download data

(Submitter supplied) In order to define the AadR regulon we did transcriptome analysis of an aadR in frame-deletion mutant and compared to that of wild type. We found that AadR positively modulated expression of 43 genes, most of which are involved in degradation of aromatic acids under anaerobic conditions. It also activated expression of four genes encoding for unknown proteins and a possible DNA-binding stress protein. more...

Organism:

Rhodopseudomonas palustris

Type:

Expression profiling by array

Platform:

GPL3953

6 Samples

Download data: CEL

(Submitter supplied) Facultative phototrophic bacteria are excellent models for analyzing the coordination of major metabolic traits including oxidative phosphorylation, photophosphorylation, carbon dioxide fixation and nitrogen fixation. In Rhodobacter sphaeroides and R. capsulatus, a two-component system called RegBA (PrrBA) controls these functions and it has been thought that this redox sensing regulatory system was essential for coordinating electron flow and could not be easily replaced in facultative phototrophs. more...

Organism:

Rhodopseudomonas palustris

Type:

Expression profiling by array

Platform:

GPL2697

9 Samples

Download data: TIFF

(Submitter supplied) A whole genome DNA microarray was used to undertake a global transcriptional analysis of nitrogen fixation and ammonium repression in Pseudomonas stutzeri A1501. The aim of this study was to identify the genes that are up-regulated under nitrogen fixation conditions and rapidly down-regulated as soon as 10 min after ammonia shock. The expression changed genes may be the candidate genes for the ammonia signal transmission or be involved in the nitrogen regulatory mechanism.

Organism:

Stutzerimonas stutzeri A1501

Type:

Expression profiling by array

Platform:

GPL4677

6 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of Rhodopseudomonas palustris (R. palustris) comparing cbbT1 over-expressing strain with cbbT2 over-expressing strain. Goal was to discriminate the molecular mechanisms between transketolase I (cbbT1) and transketolase II (cbbT2). R.palustris is a purple non-sulfur anoxygenic phototrophic bacterium and transketolase (cbbT1 and cbbT2) is a key enzyme involved in the CBB cycle. more...

Organism:

Rhodopseudomonas palustris CGA009; Rhodopseudomonas palustris

Type:

Expression profiling by array

Platform:

GPL13538

4 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling in Pseudomonas aeruginosa MPAO1 and its glyoxylate shunt gens transposon insertion mutants (aceA and glcB) was perfomed using microarray analysis with or without 1mM PQ treatment. Based on the gene expression, we investigated role of glyoxylate shunt in P. aeruginosa and found out pathway related with glyoxylate shunt under oxidative stress.

Organism:

Pseudomonas aeruginosa PAO1

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21507

12 Samples

Download data: GPR

(Submitter supplied) Rhodopseudomonas palustris strain SA008.1.07 can use syringic acid as sole organic carbon source anaerobically. Grew all anaerobically in various carbon sources: syringic acid, succinate, and p-hydroxybenzoic acid.

Organism:

Rhodopseudomonas palustris

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL27030 GPL27031

9 Samples

Download data: TXT