| Motoneuron (culture) | Cortical interneuron (culture) | Motoneuron (spinal cord preparation) | Hypoglossal motoneuron (brainstem slice) | Superior colliculus interneuron (midbrain slice) | PV+ interneuron (brain slice) | Layer V pyramidal neurons (brain slice) | |
|---|---|---|---|---|---|---|---|
| Intrinsic excitability | Reduced (69) | Increased (26) | Increased (neonatal)-unchanged (pre-symptomatic)-increased (symptomatic) (70), Reduced (72, 233) | Increased ((72, 73) | |||
| GABA-induced current | Unchanged (75, 76) | ||||||
| GABA-induced current decay τ | Unchanged (75, 76) | ||||||
| GABAergic mIPSC | Unchanged (76) | Reduced (34) | Unchanged (26) | Unchanged (26) | Unchanged (72) | Reduced (72), Unchanged (73) | |
| GABAergic mIPSC decay τ | Unchanged (75, 76) | Longer (34) | Longer (73) | ||||
| GABAergic mIPSC frequency | Unchanged (75, 76) | Reduced (34) | Increased (26) | Increased (26) | Unchanged (72) | Reduced (72, 73) | |
| GABAergic sIPSC frequency | Increased (72), Reduced (233) | Reduced (73) | |||||
| GABAA receptor desensitization | Reduced (75) | ||||||
| Glycine-induced current | Reduced (76) | ||||||
| Glycine-induced current decay τ | Unchanged (76) | ||||||
| Glycinergic mIPSC | Reduced (76) | Reduced (34), Unchanged (Zebrafish) (85) | Unchanged (26) | ||||
| Glycinergic mIPSC decay τ | Unchanged (76) | Longer (34, 85) | |||||
| Glycinergic mIPSC frequency | Unchanged (75, 76) | Reduced (34, 85) | Increased (26) | ||||
| EGABAAR | Depolarized (34) | ||||||
| [Cl-]i | Increased (34) |
Spinal embryonic neurons were isolated from E12–14 mice and cultured for 12–16 days (76), or from E15 and cultured for 8–10 days (75). Embryonic spinal cord preparations were obtained from E17.5 mice (25, 34). Brainstem slices were obtained from P4-P10 mice and midbrain slices obtained from P10-P12 mice (26). Cortical interneurons were isolated from E15.5 mice and cultured for 12 days (69). VP+ inhibitory interneurons (Gad67-GFP) were recorded in brain slices of SOD1G93A (and control) at 6 (neonatal), 26–35 days (pre-symptomatic) and 90–101 days (symptomatic) of age (70). Brain slices were obtained from P3 TDP-43A315T mice (74), or from P9 SOD1G93A mice (235). Layer 5 pyramidal neurons in brain slices from P15-P25 wobbler mice (76). Exposed spinal cord was prepared from 4 days post-fertilization larvae Sod1G93R zebrafish (87). [Cl-]i, intracellular chloride concentration; EGABAAR, reversal potential for GABAAR; mIPSC, miniature inhibitory postsynaptic current; PV, parvalbumin.
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