Warning, /src/objects/seqfeat/seqfeat.asn is written in an unsupported language. File is not indexed.
--$Revision: 79603 $
--**********************************************************************
--
-- NCBI Sequence Feature elements
-- by James Ostell, 1990
-- Version 3.0 - June 1994
--
--**********************************************************************
NCBI-Seqfeat DEFINITIONS ::=
BEGIN
EXPORTS Seq-feat, Feat-id, Genetic-code, ModelEvidenceSupport;
IMPORTS Gene-ref FROM NCBI-Gene
Prot-ref FROM NCBI-Protein
Org-ref FROM NCBI-Organism
Variation-ref FROM NCBI-Variation
BioSource FROM NCBI-BioSource
RNA-ref FROM NCBI-RNA
Seq-id, Seq-loc, Giimport-id FROM NCBI-Seqloc
Pubdesc, Numbering, Heterogen FROM NCBI-Sequence
Rsite-ref FROM NCBI-Rsite
Txinit FROM NCBI-TxInit
DOI, PubMedId FROM NCBI-Biblio
Pub-set FROM NCBI-Pub
Object-id, Dbtag, User-object FROM NCBI-General;
--*** Feature identifiers ********************************
--*
Feat-id ::= CHOICE {
gibb INTEGER , -- geninfo backbone
giim Giimport-id , -- geninfo import
local Object-id , -- for local software use
general Dbtag } -- for use by various databases
--*** Seq-feat *******************************************
--* sequence feature generalization
Seq-feat ::= SEQUENCE {
id Feat-id OPTIONAL ,
data SeqFeatData , -- the specific data
partial BOOLEAN OPTIONAL , -- incomplete in some way?
except BOOLEAN OPTIONAL , -- something funny about this?
comment VisibleString OPTIONAL ,
product Seq-loc OPTIONAL , -- product of process
location Seq-loc , -- feature made from
qual SEQUENCE OF Gb-qual OPTIONAL , -- qualifiers
title VisibleString OPTIONAL , -- for user defined label
ext User-object OPTIONAL , -- user defined structure extension
cit Pub-set OPTIONAL , -- citations for this feature
exp-ev ENUMERATED { -- evidence for existence of feature
experimental (1) , -- any reasonable experimental check
not-experimental (2) } OPTIONAL , -- similarity, pattern, etc
xref SET OF SeqFeatXref OPTIONAL , -- cite other relevant features
dbxref SET OF Dbtag OPTIONAL , -- support for xref to other databases
pseudo BOOLEAN OPTIONAL , -- annotated on pseudogene?
except-text VisibleString OPTIONAL , -- explain if except=TRUE
ids SET OF Feat-id OPTIONAL , -- set of Ids; will replace 'id' field
exts SET OF User-object OPTIONAL , -- set of extensions; will replace 'ext' field
support SeqFeatSupport OPTIONAL -- will replace /experiment, /inference, model-evidence
}
SeqFeatData ::= CHOICE {
gene Gene-ref ,
org Org-ref ,
cdregion Cdregion ,
prot Prot-ref ,
rna RNA-ref ,
pub Pubdesc , -- publication applies to this seq
seq Seq-loc , -- to annotate origin from another seq
imp Imp-feat ,
region VisibleString, -- named region (globin locus)
comment NULL , -- just a comment
bond ENUMERATED {
disulfide (1) ,
thiolester (2) ,
xlink (3) ,
thioether (4) ,
other (255) } ,
site ENUMERATED {
active (1) ,
binding (2) ,
cleavage (3) ,
inhibit (4) ,
modified (5),
glycosylation (6) ,
myristoylation (7) ,
mutagenized (8) ,
metal-binding (9) ,
phosphorylation (10) ,
acetylation (11) ,
amidation (12) ,
methylation (13) ,
hydroxylation (14) ,
sulfatation (15) ,
oxidative-deamination (16) ,
pyrrolidone-carboxylic-acid (17) ,
gamma-carboxyglutamic-acid (18) ,
blocked (19) ,
lipid-binding (20) ,
np-binding (21) ,
dna-binding (22) ,
signal-peptide (23) ,
transit-peptide (24) ,
transmembrane-region (25) ,
nitrosylation (26) ,
other (255) } ,
rsite Rsite-ref , -- restriction site (for maps really)
user User-object , -- user defined structure
txinit Txinit , -- transcription initiation
num Numbering , -- a numbering system
psec-str ENUMERATED { -- protein secondary structure
helix (1) , -- any helix
sheet (2) , -- beta sheet
turn (3) } , -- beta or gamma turn
non-std-residue VisibleString , -- non-standard residue here in seq
het Heterogen , -- cofactor, prosthetic grp, etc, bound to seq
biosrc BioSource,
clone Clone-ref,
variation Variation-ref
}
SeqFeatXref ::= SEQUENCE { -- both optional because can have one or both
id Feat-id OPTIONAL , -- the feature copied
data SeqFeatData OPTIONAL } -- the specific data
SeqFeatSupport ::= SEQUENCE {
experiment SET OF ExperimentSupport OPTIONAL ,
inference SET OF InferenceSupport OPTIONAL ,
model-evidence SET OF ModelEvidenceSupport OPTIONAL
}
EvidenceCategory ::= INTEGER {
not-set (0) ,
coordinates (1) ,
description (2) ,
existence (3)
}
ExperimentSupport ::= SEQUENCE {
category EvidenceCategory OPTIONAL ,
explanation VisibleString ,
pmids SET OF PubMedId OPTIONAL ,
dois SET OF DOI OPTIONAL
}
Program-id ::= SEQUENCE {
name VisibleString ,
version VisibleString OPTIONAL
}
EvidenceBasis ::= SEQUENCE {
programs SET OF Program-id OPTIONAL ,
accessions SET OF Seq-id OPTIONAL
}
InferenceSupport ::= SEQUENCE {
category EvidenceCategory OPTIONAL ,
type INTEGER {
not-set (0) ,
similar-to-sequence (1) ,
similar-to-aa (2) ,
similar-to-dna (3) ,
similar-to-rna (4) ,
similar-to-mrna (5) ,
similiar-to-est (6) ,
similar-to-other-rna (7) ,
profile (8) ,
nucleotide-motif (9) ,
protein-motif (10) ,
ab-initio-prediction (11) ,
alignment (12) ,
other (255)
} DEFAULT not-set ,
other-type VisibleString OPTIONAL ,
same-species BOOLEAN DEFAULT FALSE ,
basis EvidenceBasis ,
pmids SET OF PubMedId OPTIONAL ,
dois SET OF DOI OPTIONAL
}
ModelEvidenceItem ::= SEQUENCE {
id Seq-id ,
exon-count INTEGER OPTIONAL ,
exon-length INTEGER OPTIONAL ,
full-length BOOLEAN DEFAULT FALSE ,
supports-all-exon-combo BOOLEAN DEFAULT FALSE
}
ModelEvidenceSupport ::= SEQUENCE {
method VisibleString OPTIONAL ,
mrna SET OF ModelEvidenceItem OPTIONAL ,
est SET OF ModelEvidenceItem OPTIONAL ,
protein SET OF ModelEvidenceItem OPTIONAL ,
identification Seq-id OPTIONAL ,
dbxref SET OF Dbtag OPTIONAL ,
exon-count INTEGER OPTIONAL ,
exon-length INTEGER OPTIONAL ,
full-length BOOLEAN DEFAULT FALSE ,
supports-all-exon-combo BOOLEAN DEFAULT FALSE
}
--*** CdRegion ***********************************************
--*
--* Instructions to translate from a nucleic acid to a peptide
--* conflict means it's supposed to translate but doesn't
--*
Cdregion ::= SEQUENCE {
orf BOOLEAN OPTIONAL , -- just an ORF ?
frame ENUMERATED {
not-set (0) , -- not set, code uses one
one (1) ,
two (2) ,
three (3) } DEFAULT not-set , -- reading frame
conflict BOOLEAN OPTIONAL , -- conflict
gaps INTEGER OPTIONAL , -- number of gaps on conflict/except
mismatch INTEGER OPTIONAL , -- number of mismatches on above
code Genetic-code OPTIONAL , -- genetic code used
code-break SEQUENCE OF Code-break OPTIONAL , -- individual exceptions
stops INTEGER OPTIONAL } -- number of stop codons on above
-- each code is 64 cells long, in the order where
-- T=0,C=1,A=2,G=3, TTT=0, TTC=1, TCA=4, etc
-- NOTE: this order does NOT correspond to a Seq-data
-- encoding. It is "natural" to codon usage instead.
-- the value in each cell is the AA coded for
-- start= AA coded only if first in peptide
-- in start array, if codon is not a legitimate start
-- codon, that cell will have the "gap" symbol for
-- that alphabet. Otherwise it will have the AA
-- encoded when that codon is used at the start.
Genetic-code ::= SET OF CHOICE {
name VisibleString , -- name of a code
id INTEGER , -- id in dbase
ncbieaa VisibleString , -- indexed to IUPAC extended
ncbi8aa OCTET STRING , -- indexed to NCBI8aa
ncbistdaa OCTET STRING , -- indexed to NCBIstdaa
sncbieaa VisibleString , -- start, indexed to IUPAC extended
sncbi8aa OCTET STRING , -- start, indexed to NCBI8aa
sncbistdaa OCTET STRING } -- start, indexed to NCBIstdaa
Code-break ::= SEQUENCE { -- specific codon exceptions
loc Seq-loc , -- location of exception
aa CHOICE { -- the amino acid
ncbieaa INTEGER , -- ASCII value of NCBIeaa code
ncbi8aa INTEGER , -- NCBI8aa code
ncbistdaa INTEGER } } -- NCBIstdaa code
Genetic-code-table ::= SET OF Genetic-code -- table of genetic codes
--*** Import ***********************************************
--*
--* Features imported from other databases
--*
Imp-feat ::= SEQUENCE {
key VisibleString ,
loc VisibleString OPTIONAL , -- original location string
descr VisibleString OPTIONAL } -- text description
Gb-qual ::= SEQUENCE {
qual VisibleString ,
val VisibleString }
--*** Clone-ref ***********************************************
--*
--* Specification of clone features
--*
Clone-ref ::= SEQUENCE {
name VisibleString, -- Official clone symbol
library VisibleString OPTIONAL, -- Library name
concordant BOOLEAN DEFAULT FALSE, -- OPTIONAL?
unique BOOLEAN DEFAULT FALSE, -- OPTIONAL?
placement-method INTEGER {
end-seq (0), -- Clone placed by end sequence
insert-alignment (1), -- Clone placed by insert alignment
sts (2), -- Clone placed by STS
fish (3),
fingerprint (4),
end-seq-insert-alignment (5), -- combined end-seq and insert align
external (253), -- Placement provided externally
curated (254), -- Human placed or approved
other (255)
} OPTIONAL,
clone-seq Clone-seq-set OPTIONAL
}
Clone-seq-set ::= SET OF Clone-seq
Clone-seq ::= SEQUENCE {
type INTEGER {
insert (0),
end (1),
other (255)
},
confidence INTEGER {
multiple (0), -- Multiple hits
na (1), -- Unspecified
nohit-rep (2), -- No hits, end flagged repetitive
nohitnorep (3), -- No hits, end not flagged repetitive
other-chrm (4), -- Hit on different chromosome
unique (5),
virtual (6), -- Virtual (hasn't been sequenced)
multiple-rep (7), -- Multiple hits, end flagged repetitive
multiplenorep (8), -- Multiple hits, end not flagged repetitive
no-hit (9), -- No hits
other (255)
} OPTIONAL,
location Seq-loc, -- location on sequence
seq Seq-loc OPTIONAL, -- clone sequence location
align-id Dbtag OPTIONAL, -- internal alignment identifier
support INTEGER {
prototype (0), -- sequence used to place clone
supporting (1), -- sequence supports placement
supports-other(2), -- supports a different placement
non-supporting (3) -- does not support any placement
} OPTIONAL
}
END
--*** Variation-ref ***********************************************
--*
--* Specification of variation features
--*
NCBI-Variation DEFINITIONS ::=
BEGIN
EXPORTS Variation-ref, Variation-inst, VariantProperties,
Population-data, Phenotype;
IMPORTS Int-fuzz, User-object, Object-id, Dbtag FROM NCBI-General
Seq-literal FROM NCBI-Sequence
SubSource FROM NCBI-BioSource
Seq-loc FROM NCBI-Seqloc
Pub FROM NCBI-Pub;
-- --------------------------------------------------------------------------
-- Historically, the dbSNP definitions document data structures used in the
-- processing and annotation of variations by the dbSNP group. The intention
-- is to provide information to clients that reflect internal information
-- produced during the mapping of SNPs
-- --------------------------------------------------------------------------
VariantProperties ::= SEQUENCE {
version INTEGER,
-- NOTE:
-- The format for most of these values is as an integer
-- Unless otherwise noted, these integers represent a bitwise OR (= simple
-- sum) of the possible values, and as such, these values represent the
-- specific bit flags that may be set for each of the possible attributes
-- here.
resource-link INTEGER {
preserved (1), -- Clinical, Pubmed, Cited, (0x01)
provisional (2), -- Provisional Third Party Annotations (0x02)
has3D (4), -- Has 3D strcture SNP3D table (0x04)
submitterLinkout (8), -- SNP->SubSNP->Batch link_out (0x08)
clinical (16), -- Clinical if LSDB, OMIM, TPA, Diagnostic (0x10)
genotypeKit (32) -- Marker exists on high density genotyping kit
-- (0x20)
} OPTIONAL,
gene-location INTEGER {
in-gene (1), -- Sequence intervals covered by a gene ID but not
-- having an aligned transcript (0x01)
near-gene-5 (2), -- Within 2kb of the 5' end of a gene feature
near-gene-3 (4), -- Within 0.5kb of the 3' end of a gene feature
intron (8), -- In Intron (0x08)
donor (16), -- In donor splice-site (0x10)
acceptor (32), -- In acceptor splice-site (0x20)
utr-5 (64), -- In 5' UTR (0x40)
utr-3 (128), -- In 3' UTR (0x80)
in-start-codon(256), -- the variant is observed in a start codon
-- (0x100)
in-stop-codon (512), -- the variant is observed in a stop codon
-- (0x200)
intergenic (1024), -- variant located between genes (0x400)
conserved-noncoding(2048) -- variant is located in a conserved
-- non-coding region (0x800)
} OPTIONAL,
effect INTEGER {
no-change (0), -- known to cause no functional changes
-- since 0 does not combine with any other bit
-- value, 'no-change' specifically implies that
-- there are no consequences
synonymous (1), -- one allele in the set does not change the encoded
-- amino acid (0x1)
nonsense (2), -- one allele in the set changes to STOP codon
-- (TER). (0x2)
missense (4), -- one allele in the set changes protein peptide
-- (0x4)
frameshift (8), -- one allele in the set changes all downstream
-- amino acids (0x8)
up-regulator (16), -- the variant causes increased transcription
-- (0x10)
down-regulator(32), -- the variant causes decreased transcription
-- (0x20)
methylation (64),
stop-gain (128), -- reference codon is not stop codon, but the snp
-- variant allele changes the codon to a
-- terminating codon.
stop-loss (256) -- reverse of STOP-GAIN: reference codon is a
-- stop codon, but a snp variant allele changes
-- the codon to a non-terminating codon.
} OPTIONAL,
mapping INTEGER {
has-other-snp (1), -- Another SNP has the same mapped positions
-- on reference assembly (0x01)
has-assembly-conflict (2), -- Weight 1 or 2 SNPs that map to different
-- chromosomes on different assemblies (0x02)
is-assembly-specific (4) -- Only maps to 1 assembly (0x04)
} OPTIONAL,
-- map-weight captures specificity of placement
-- NOTE: This is *NOT* a bitfield
map-weight INTEGER {
is-uniquely-placed(1),
placed-twice-on-same-chrom(2),
placed-twice-on-diff-chrom(3),
many-placements(10)
} OPTIONAL,
frequency-based-validation INTEGER {
is-mutation (1), -- low frequency variation that is cited in
-- journal or other reputable sources (0x01)
above-5pct-all (2), -- >5% minor allele freq in each and all
-- populations (0x02)
above-5pct-1plus (4), -- >5% minor allele freq in 1+ populations (0x04)
validated (8), -- Bit is set if the variant has a minor allele
-- observed in two or more separate chromosomes
above-1pct-all (16), -- >1% minor allele freq in each and all
-- populations (0x10)
above-1pct-1plus (32) -- >1% minor allele freq in 1+ populations (0x20)
} OPTIONAL,
genotype INTEGER {
in-haplotype-set (1), -- Exists in a haplotype tagging set (0x01)
has-genotypes (2) -- SNP has individual genotype (0x02)
} OPTIONAL,
-- project IDs are IDs from BioProjects
-- in order to report information about project relationships, we
-- require projects to be registered
-- This field in many ways duplicates dbxrefs; however, the
-- intention of this field is to more adequately reflect
-- ownership and data source
--
-- 11/9/2010: DO NOT USE
-- This field was changed in the spec in a breaking way; using it will
-- break clients. We are officially suppressing / abandoning this field.
-- Clients who need to use this should instead place the data in
-- Seq-feat.dbxref, using the db name 'BioProject'
project-data SET OF INTEGER OPTIONAL,
quality-check INTEGER {
contig-allele-missing (1), -- Reference sequence allele at the mapped
-- position is not present in the SNP
-- allele list, adjusted for orientation
-- (0x01)
withdrawn-by-submitter (2), -- One member SS is withdrawn by submitter
-- (0x02)
non-overlapping-alleles (4), -- RS set has 2+ alleles from different
-- submissions and these sets share no
-- alleles in common (0x04)
strain-specific (8), -- Straing specific fixed difference (0x08)
genotype-conflict (16) -- Has Genotype Conflict (0x10)
} OPTIONAL,
confidence INTEGER {
unknown (0),
likely-artifact (1),
other (255)
} OPTIONAL,
-- has this variant been validated?
-- While a boolean flag offers no subtle distinctions of validation
-- methods, occasionally it is only known as a single boolean value
-- NOTE: this flag is redundant and should be omitted if more comprehensive
-- validation information is present
other-validation BOOLEAN OPTIONAL,
-- origin of this allele, if known
-- note that these are powers-of-two, and represent bits; thus, we can
-- represent more than one state simultaneously through a bitwise OR
allele-origin INTEGER {
unknown (0),
germline (1),
somatic (2),
inherited (4),
paternal (8),
maternal (16),
de-novo (32),
biparental (64),
uniparental (128),
not-tested (256),
tested-inconclusive (512),
not-reported (1024),
-- stopper - 2^31
other (1073741824)
} OPTIONAL,
-- observed allele state, if known
-- NOTE: THIS IS NOT A BITFIELD!
allele-state INTEGER {
unknown (0),
homozygous (1),
heterozygous (2),
hemizygous (3),
nullizygous (4),
other (255)
} OPTIONAL,
-- NOTE:
-- 'allele-frequency' here refers to the minor allele frequency of the
-- default population
allele-frequency REAL OPTIONAL,
-- is this variant the ancestral allele?
is-ancestral-allele BOOLEAN OPTIONAL
}
Phenotype ::= SEQUENCE {
source VisibleString OPTIONAL,
term VisibleString OPTIONAL,
xref SET OF Dbtag OPTIONAL,
-- does this variant have known clinical significance?
clinical-significance INTEGER {
unknown (0),
untested (1),
non-pathogenic (2),
probable-non-pathogenic (3),
probable-pathogenic (4),
pathogenic (5),
drug-response (6),
histocompatibility (7),
other (255)
} OPTIONAL
}
Population-data ::= SEQUENCE {
-- assayed population (e.g. HAPMAP-CEU)
population VisibleString,
genotype-frequency REAL OPTIONAL,
chromosomes-tested INTEGER OPTIONAL,
sample-ids SET OF Object-id OPTIONAL,
allele-frequency REAL OPTIONAL,
-- This field is an explicit bit-field
-- Valid values should be a bitwise combination (= simple sum)
-- of any of the values below
flags INTEGER {
is-default-population (1),
is-minor-allele (2),
is-rare-allele (4)
} OPTIONAL
}
Ext-loc ::= SEQUENCE {
id Object-id,
location Seq-loc
}
Variation-ref ::= SEQUENCE {
-- ids (i.e., SNP rsid / ssid, dbVar nsv/nssv)
-- expected values include 'dbSNP|rs12334', 'dbSNP|ss12345', 'dbVar|nsv1'
--
-- we relate three kinds of IDs here:
-- - our current object's id
-- - the id of this object's parent, if it exists
-- - the sample ID that this item originates from
id Dbtag OPTIONAL,
parent-id Dbtag OPTIONAL,
sample-id Object-id OPTIONAL,
other-ids SET OF Dbtag OPTIONAL,
-- names and synonyms
-- some variants have well-known canonical names and possible accepted
-- synonyms
name VisibleString OPTIONAL,
synonyms SET OF VisibleString OPTIONAL,
-- tag for comment and descriptions
description VisibleString OPTIONAL,
-- phenotype
phenotype SET OF Phenotype OPTIONAL,
-- sequencing / acuisition method
method SET OF INTEGER {
unknown (0),
bac-acgh (1),
computational (2),
curated (3),
digital-array (4),
expression-array (5),
fish (6),
flanking-sequence (7),
maph (8),
mcd-analysis (9),
mlpa (10),
oea-assembly (11),
oligo-acgh (12),
paired-end (13),
pcr (14),
qpcr (15),
read-depth (16),
roma (17),
rt-pcr (18),
sage (19),
sequence-alignment (20),
sequencing (21),
snp-array (22),
snp-genoytyping (23),
southern (24),
western (25),
optical-mapping (26),
other (255)
} OPTIONAL,
-- Note about SNP representation and pretinent fields: allele-frequency,
-- population, quality-codes:
-- The case of multiple alleles for a SNP would be described by
-- parent-feature of type Variation-set.diff-alleles, where the child
-- features of type Variation-inst, all at the same location, would
-- describe individual alleles.
-- population data
-- DEPRECATED - do not use
population-data SET OF Population-data OPTIONAL,
-- variant properties bit fields
variant-prop VariantProperties OPTIONAL,
-- has this variant been validated?
-- DEPRECATED: new field = VariantProperties.other-validation
validated BOOLEAN OPTIONAL,
-- link-outs to GeneTests database
-- DEPRECATED - do not use
clinical-test SET OF Dbtag OPTIONAL,
-- origin of this allele, if known
-- note that these are powers-of-two, and represent bits; thus, we can
-- represent more than one state simultaneously through a bitwise OR
-- DEPRECATED: new field = VariantProperties.allele-origin
allele-origin INTEGER {
unknown (0),
germline (1),
somatic (2),
inherited (4),
paternal (8),
maternal (16),
de-novo (32),
biparental (64),
uniparental (128),
not-tested (256),
tested-inconclusive (512),
-- stopper - 2^31
other (1073741824)
} OPTIONAL,
-- observed allele state, if known
-- DEPRECATED: new field = VariantProperties.allele-state
allele-state INTEGER {
unknown (0),
homozygous (1),
heterozygous (2),
hemizygous (3),
nullizygous (4),
other (255)
} OPTIONAL,
-- NOTE:
-- 'allele-frequency' here refers to the minor allele frequency of the
-- default population
-- DEPRECATED: new field = VariantProperties.allele-frequency
allele-frequency REAL OPTIONAL,
-- is this variant the ancestral allele?
-- DEPRECATED: new field = VariantProperties.is-ancestral-allele
is-ancestral-allele BOOLEAN OPTIONAL,
-- publication support.
-- Note: made this pub instead of pub-equiv, since
-- Pub can be pub-equiv and pub-equiv is a set of pubs, but it looks like
-- Pub is more often used as top-level container
-- DEPRECATED - do not use; use Seq-feat.dbxref instead
pub Pub OPTIONAL,
data CHOICE {
unknown NULL,
note VisibleString, --free-form
uniparental-disomy NULL,
-- actual sequence-edit at feat.location
instance Variation-inst,
-- Set of related Variations.
-- Location of the set equals to the union of member locations
set SEQUENCE {
type INTEGER {
unknown (0),
compound (1), -- complex change at the same location on the
-- same molecule
products (2), -- different products arising from the same
-- variation in a precursor, e.g. r.[13g>a,
-- 13_88del]
haplotype (3), -- changes on the same allele, e.g
-- r.[13g>a;15u>c]
genotype (4), -- changes on different alleles in the same
-- genotype, e.g. g.[476C>T]+[476C>T]
mosaic (5), -- different genotypes in the same individual
individual (6), -- same organism; allele relationship unknown,
-- e.g. g.[476C>T(+)183G>C]
population (7), -- population
alleles (8), -- set represents a set of observed alleles
package (9), -- set represents a package of observations at
-- a given location, generally containing
-- asserted + reference
other (255)
},
variations SET OF Variation-ref,
name VisibleString OPTIONAL
},
-- variant is a complex and undescribed change at the location
-- This type of variant is known to occur in dbVar submissions
complex NULL
},
consequence SET OF CHOICE {
unknown NULL,
splicing NULL, --some effect on splicing
note VisibleString, --freeform
-- Describe resulting variation in the product, e.g. missense,
-- nonsense, silent, neutral, etc in a protein, that arises from
-- THIS variation.
variation Variation-ref,
-- see http://www.hgvs.org/mutnomen/recs-prot.html
frameshift SEQUENCE {
phase INTEGER OPTIONAL,
x-length INTEGER OPTIONAL
},
loss-of-heterozygosity SEQUENCE {
-- In germline comparison, it will be reference genome assembly
-- (default) or reference/normal population. In somatic mutation,
-- it will be a name of the normal tissue.
reference VisibleString OPTIONAL,
-- Name of the testing subject type or the testing tissue.
test VisibleString OPTIONAL
}
} OPTIONAL,
-- Observed location, if different from the parent set or feature.location.
-- DEPRECATED - do not use
location Seq-loc OPTIONAL,
-- reference other locs, e.g. mapped source
-- DEPRECATED - do not use
ext-locs SET OF Ext-loc OPTIONAL,
-- DEPRECATED - do not use; use Seq-feat.exts instead
ext User-object OPTIONAL,
somatic-origin SET OF SEQUENCE {
-- description of the somatic origin itself
source SubSource OPTIONAL,
-- condition related to this origin's type
condition SEQUENCE {
description VisibleString OPTIONAL,
-- reference to BioTerm / other descriptive database
object-id SET OF Dbtag OPTIONAL
} OPTIONAL
} OPTIONAL
}
Delta-item ::= SEQUENCE {
seq CHOICE {
literal Seq-literal,
loc Seq-loc,
this NULL --same location as variation-ref itself
} OPTIONAL,
-- Multiplier allows representing a tandem, e.g. ATATAT as AT*3
-- This allows describing CNV/SSR where delta=self with a
-- multiplier which specifies the count of the repeat unit.
multiplier INTEGER OPTIONAL, --assumed 1 if not specified.
multiplier-fuzz Int-fuzz OPTIONAL,
action INTEGER {
-- replace len(seq) positions starting with location.start with seq
morph (0),
-- go downstream by distance specified by multiplier (upstream if < 0),
-- in genomic context.
offset (1),
-- excise sequence at location
-- if multiplier is specified, delete len(location)*multiplier
-- positions downstream
del-at (2),
-- insert seq before the location.start
ins-before (3)
} DEFAULT morph
}
-- Variation instance
Variation-inst ::= SEQUENCE {
type INTEGER {
unknown (0), -- delta=[]
identity (1), -- delta=[]
inv (2), -- delta=[del, ins.seq=
-- RevComp(variation-location)]
snv (3), -- delta=[morph of length 1]
-- NOTE: this is snV not snP; the latter
-- requires frequency-based validation to be
-- established in VariantProperties
-- the strict definition of SNP is an SNV with
-- an established population frequency of at
-- least 1% in at least 1 popuplation
mnp (4), -- delta=[morph of length >1]
delins (5), -- delta=[del, ins]
del (6), -- delta=[del]
ins (7), -- delta=[ins]
microsatellite (8), -- delta=[del, ins.seq= repeat-unit with fuzzy
-- multiplier]
-- variation-location is the microsat expansion
-- on the sequence
transposon (9), -- delta=[del, ins.seq= known donor or 'this']
-- variation-location is equiv of transposon
-- locs.
cnv (10), -- delta=[del, ins= 'this' with fuzzy
-- multiplier]
direct-copy (11), -- delta=[ins.seq= upstream location on the
-- same strand]
rev-direct-copy (12), -- delta=[ins.seq= downstream location on the
-- same strand]
inverted-copy (13), -- delta=[ins.seq= upstream location on the
-- opposite strand]
everted-copy (14), -- delta=[ins.seq= downstream location on the
-- opposite strand]
translocation (15), -- delta=like delins
prot-missense (16), -- delta=[morph of length 1]
prot-nonsense (17), -- delta=[del]; variation-location is the tail
-- of the protein being truncated
prot-neutral (18), -- delta=[morph of length 1]
prot-silent (19), -- delta=[morph of length 1, same AA as at
-- variation-location]
prot-other (20), -- delta=any
other (255) -- delta=any
},
-- Sequence that replaces the location, in biological order.
delta SEQUENCE OF Delta-item,
-- 'observation' is used to label items in a Variation-ref package
-- This field is explicitly a bit-field, so the bitwise OR (= sum) of any
-- of the values may be observed.
observation INTEGER {
asserted (1), -- inst represents the asserted base at a
-- position
reference (2), -- inst represents the reference base at the
-- position
variant (4) -- inst represent the observed variant at a
-- given position
} OPTIONAL
}
END
--**********************************************************************
--
-- NCBI Restriction Sites
-- by James Ostell, 1990
-- version 0.8
--
--**********************************************************************
NCBI-Rsite DEFINITIONS ::=
BEGIN
EXPORTS Rsite-ref;
IMPORTS Dbtag FROM NCBI-General;
Rsite-ref ::= CHOICE {
str VisibleString , -- may be unparsable
db Dbtag } -- pointer to a restriction site database
END
--**********************************************************************
--
-- NCBI RNAs
-- by James Ostell, 1990
-- version 0.8
--
--**********************************************************************
NCBI-RNA DEFINITIONS ::=
BEGIN
EXPORTS RNA-ref, Trna-ext, RNA-gen, RNA-qual, RNA-qual-set;
IMPORTS Seq-loc FROM NCBI-Seqloc;
--*** rnas ***********************************************
--*
--* various rnas
--*
-- minimal RNA sequence
RNA-ref ::= SEQUENCE {
type ENUMERATED { -- type of RNA feature
unknown (0) ,
premsg (1) ,
mRNA (2) ,
tRNA (3) ,
rRNA (4) ,
snRNA (5) , -- will become ncRNA, with RNA-gen.class = snRNA
scRNA (6) , -- will become ncRNA, with RNA-gen.class = scRNA
snoRNA (7) , -- will become ncRNA, with RNA-gen.class = snoRNA
ncRNA (8) , -- non-coding RNA; subsumes snRNA, scRNA, snoRNA
tmRNA (9) ,
miscRNA (10) ,
other (255) } ,
pseudo BOOLEAN OPTIONAL ,
ext CHOICE {
name VisibleString , -- for naming "other" type
tRNA Trna-ext , -- for tRNAs
gen RNA-gen } OPTIONAL -- generic fields for ncRNA, tmRNA, miscRNA
}
Trna-ext ::= SEQUENCE { -- tRNA feature extensions
aa CHOICE { -- aa this carries
iupacaa INTEGER ,
ncbieaa INTEGER ,
ncbi8aa INTEGER ,
ncbistdaa INTEGER } OPTIONAL ,
codon SET OF INTEGER OPTIONAL , -- codon(s) as in Genetic-code
anticodon Seq-loc OPTIONAL } -- location of anticodon
RNA-gen ::= SEQUENCE {
class VisibleString OPTIONAL , -- for ncRNAs, the class of non-coding RNA:
-- examples: antisense_RNA, guide_RNA, snRNA
product VisibleString OPTIONAL ,
quals RNA-qual-set OPTIONAL -- e.g., tag_peptide qualifier for tmRNAs
}
RNA-qual ::= SEQUENCE { -- Additional data values for RNA-gen,
qual VisibleString , -- in a tag (qual), value (val) format
val VisibleString }
RNA-qual-set ::= SEQUENCE OF RNA-qual
END
--**********************************************************************
--
-- NCBI Genes
-- by James Ostell, 1990
-- version 0.8
--
--**********************************************************************
NCBI-Gene DEFINITIONS ::=
BEGIN
EXPORTS Gene-ref, Gene-nomenclature;
IMPORTS Dbtag FROM NCBI-General;
--*** Gene ***********************************************
--*
--* reference to a gene
--*
Gene-ref ::= SEQUENCE {
locus VisibleString OPTIONAL , -- Official gene symbol
allele VisibleString OPTIONAL , -- Official allele designation
desc VisibleString OPTIONAL , -- descriptive name
maploc VisibleString OPTIONAL , -- descriptive map location
pseudo BOOLEAN DEFAULT FALSE , -- pseudogene
db SET OF Dbtag OPTIONAL , -- ids in other dbases
syn SET OF VisibleString OPTIONAL , -- synonyms for locus
locus-tag VisibleString OPTIONAL , -- systematic gene name (e.g., MI0001, ORF0069)
formal-name Gene-nomenclature OPTIONAL
}
Gene-nomenclature ::= SEQUENCE {
status ENUMERATED {
unknown (0) ,
official (1) ,
interim (2)
} ,
symbol VisibleString OPTIONAL ,
name VisibleString OPTIONAL ,
source Dbtag OPTIONAL
}
END
--**********************************************************************
--
-- NCBI Organism
-- by James Ostell, 1994
-- version 3.0
--
--**********************************************************************
NCBI-Organism DEFINITIONS ::=
BEGIN
EXPORTS Org-ref;
IMPORTS Dbtag FROM NCBI-General;
--*** Org-ref ***********************************************
--*
--* Reference to an organism
--* defines only the organism.. lower levels of detail for biological
--* molecules are provided by the Source object
--*
Org-ref ::= SEQUENCE {
taxname VisibleString OPTIONAL , -- preferred formal name
common VisibleString OPTIONAL , -- common name
mod SET OF VisibleString OPTIONAL , -- unstructured modifiers
db SET OF Dbtag OPTIONAL , -- ids in taxonomic or culture dbases
syn SET OF VisibleString OPTIONAL , -- synonyms for taxname or common
orgname OrgName OPTIONAL }
OrgName ::= SEQUENCE {
name CHOICE {
binomial BinomialOrgName , -- genus/species type name
virus VisibleString , -- virus names are different
hybrid MultiOrgName , -- hybrid between organisms
namedhybrid BinomialOrgName , -- some hybrids have genus x species name
partial PartialOrgName } OPTIONAL , -- when genus not known
attrib VisibleString OPTIONAL , -- attribution of name
mod SEQUENCE OF OrgMod OPTIONAL ,
lineage VisibleString OPTIONAL , -- lineage with semicolon separators
gcode INTEGER OPTIONAL , -- genetic code (see CdRegion)
mgcode INTEGER OPTIONAL , -- mitochondrial genetic code
div VisibleString OPTIONAL , -- GenBank division code
pgcode INTEGER OPTIONAL } -- plastid genetic code
OrgMod ::= SEQUENCE {
subtype INTEGER {
strain (2) ,
substrain (3) ,
type (4) ,
subtype (5) ,
variety (6) ,
serotype (7) ,
serogroup (8) ,
serovar (9) ,
cultivar (10) ,
pathovar (11) ,
chemovar (12) ,
biovar (13) ,
biotype (14) ,
group (15) ,
subgroup (16) ,
isolate (17) ,
common (18) ,
acronym (19) ,
dosage (20) , -- chromosome dosage of hybrid
nat-host (21) , -- natural host of this specimen
sub-species (22) ,
specimen-voucher (23) ,
authority (24) ,
forma (25) ,
forma-specialis (26) ,
ecotype (27) ,
synonym (28) ,
anamorph (29) ,
teleomorph (30) ,
breed (31) ,
gb-acronym (32) , -- used by taxonomy database
gb-anamorph (33) , -- used by taxonomy database
gb-synonym (34) , -- used by taxonomy database
culture-collection (35) ,
bio-material (36) ,
metagenome-source (37) ,
type-material (38) ,
nomenclature (39) , -- code of nomenclature in subname (B,P,V,Z or combination)
old-lineage (253) ,
old-name (254) ,
other (255) } , -- ASN5: old-name (254) will be added to next spec
subname VisibleString ,
attrib VisibleString OPTIONAL } -- attribution/source of name
BinomialOrgName ::= SEQUENCE {
genus VisibleString , -- required
species VisibleString OPTIONAL , -- species required if subspecies used
subspecies VisibleString OPTIONAL }
MultiOrgName ::= SEQUENCE OF OrgName -- the first will be used to assign division
PartialOrgName ::= SEQUENCE OF TaxElement -- when we don't know the genus
TaxElement ::= SEQUENCE {
fixed-level INTEGER {
other (0) , -- level must be set in string
family (1) ,
order (2) ,
class (3) } ,
level VisibleString OPTIONAL ,
name VisibleString }
END
--**********************************************************************
--
-- NCBI BioSource
-- by James Ostell, 1994
-- version 3.0
--
--**********************************************************************
NCBI-BioSource DEFINITIONS ::=
BEGIN
EXPORTS BioSource, SubSource;
IMPORTS Org-ref FROM NCBI-Organism;
--********************************************************************
--
-- BioSource gives the source of the biological material
-- for sequences
--
--********************************************************************
BioSource ::= SEQUENCE {
genome INTEGER { -- biological context
unknown (0) ,
genomic (1) ,
chloroplast (2) ,
chromoplast (3) ,
kinetoplast (4) ,
mitochondrion (5) ,
plastid (6) ,
macronuclear (7) ,
extrachrom (8) ,
plasmid (9) ,
transposon (10) ,
insertion-seq (11) ,
cyanelle (12) ,
proviral (13) ,
virion (14) ,
nucleomorph (15) ,
apicoplast (16) ,
leucoplast (17) ,
proplastid (18) ,
endogenous-virus (19) ,
hydrogenosome (20) ,
chromosome (21) ,
chromatophore (22) ,
plasmid-in-mitochondrion (23) ,
plasmid-in-plastid (24)
} DEFAULT unknown ,
origin INTEGER {
unknown (0) ,
natural (1) , -- normal biological entity
natmut (2) , -- naturally occurring mutant
mut (3) , -- artificially mutagenized
artificial (4) , -- artificially engineered
synthetic (5) , -- purely synthetic
other (255)
} DEFAULT unknown ,
org Org-ref ,
subtype SEQUENCE OF SubSource OPTIONAL ,
is-focus NULL OPTIONAL , -- to distinguish biological focus
pcr-primers PCRReactionSet OPTIONAL }
PCRReactionSet ::= SET OF PCRReaction
PCRReaction ::= SEQUENCE {
forward PCRPrimerSet OPTIONAL ,
reverse PCRPrimerSet OPTIONAL }
PCRPrimerSet ::= SET OF PCRPrimer
PCRPrimer ::= SEQUENCE {
seq PCRPrimerSeq OPTIONAL ,
name PCRPrimerName OPTIONAL }
PCRPrimerSeq ::= VisibleString
PCRPrimerName ::= VisibleString
SubSource ::= SEQUENCE {
subtype INTEGER {
chromosome (1) ,
map (2) ,
clone (3) ,
subclone (4) ,
haplotype (5) ,
genotype (6) ,
sex (7) ,
cell-line (8) ,
cell-type (9) ,
tissue-type (10) ,
clone-lib (11) ,
dev-stage (12) ,
frequency (13) ,
germline (14) ,
rearranged (15) ,
lab-host (16) ,
pop-variant (17) ,
tissue-lib (18) ,
plasmid-name (19) ,
transposon-name (20) ,
insertion-seq-name (21) ,
plastid-name (22) ,
country (23) ,
segment (24) ,
endogenous-virus-name (25) ,
transgenic (26) ,
environmental-sample (27) ,
isolation-source (28) ,
lat-lon (29) , -- +/- decimal degrees
collection-date (30) , -- DD-MMM-YYYY format
collected-by (31) , -- name of person who collected the sample
identified-by (32) , -- name of person who identified the sample
fwd-primer-seq (33) , -- sequence (possibly more than one; semicolon-separated)
rev-primer-seq (34) , -- sequence (possibly more than one; semicolon-separated)
fwd-primer-name (35) ,
rev-primer-name (36) ,
metagenomic (37) ,
mating-type (38) ,
linkage-group (39) ,
haplogroup (40) ,
whole-replicon (41) ,
phenotype (42) ,
altitude (43) ,
other (255) } ,
name VisibleString ,
attrib VisibleString OPTIONAL } -- attribution/source of this name
END
--**********************************************************************
--
-- NCBI Protein
-- by James Ostell, 1990
-- version 0.8
--
--**********************************************************************
NCBI-Protein DEFINITIONS ::=
BEGIN
EXPORTS Prot-ref;
IMPORTS Dbtag FROM NCBI-General;
--*** Prot-ref ***********************************************
--*
--* Reference to a protein name
--*
Prot-ref ::= SEQUENCE {
name SET OF VisibleString OPTIONAL , -- protein name
desc VisibleString OPTIONAL , -- description (instead of name)
ec SET OF VisibleString OPTIONAL , -- E.C. number(s)
activity SET OF VisibleString OPTIONAL , -- activities
db SET OF Dbtag OPTIONAL , -- ids in other dbases
processed ENUMERATED { -- processing status
not-set (0) ,
preprotein (1) ,
mature (2) ,
signal-peptide (3) ,
transit-peptide (4) ,
propeptide (5) } DEFAULT not-set }
END
--********************************************************************
--
-- Transcription Initiation Site Feature Data Block
-- James Ostell, 1991
-- Philip Bucher, David Ghosh
-- version 1.1
--
--
--
--********************************************************************
NCBI-TxInit DEFINITIONS ::=
BEGIN
EXPORTS Txinit;
IMPORTS Gene-ref FROM NCBI-Gene
Prot-ref FROM NCBI-Protein
Org-ref FROM NCBI-Organism;
Txinit ::= SEQUENCE {
name VisibleString , -- descriptive name of initiation site
syn SEQUENCE OF VisibleString OPTIONAL , -- synonyms
gene SEQUENCE OF Gene-ref OPTIONAL , -- gene(s) transcribed
protein SEQUENCE OF Prot-ref OPTIONAL , -- protein(s) produced
rna SEQUENCE OF VisibleString OPTIONAL , -- rna(s) produced
expression VisibleString OPTIONAL , -- tissue/time of expression
txsystem ENUMERATED { -- transcription apparatus used at this site
unknown (0) ,
pol1 (1) , -- eukaryotic Pol I
pol2 (2) , -- eukaryotic Pol II
pol3 (3) , -- eukaryotic Pol III
bacterial (4) ,
viral (5) ,
rna (6) , -- RNA replicase
organelle (7) ,
other (255) } ,
txdescr VisibleString OPTIONAL , -- modifiers on txsystem
txorg Org-ref OPTIONAL , -- organism supplying transcription apparatus
mapping-precise BOOLEAN DEFAULT FALSE , -- mapping precise or approx
location-accurate BOOLEAN DEFAULT FALSE , -- does Seq-loc reflect mapping
inittype ENUMERATED {
unknown (0) ,
single (1) ,
multiple (2) ,
region (3) } OPTIONAL ,
evidence SET OF Tx-evidence OPTIONAL }
Tx-evidence ::= SEQUENCE {
exp-code ENUMERATED {
unknown (0) ,
rna-seq (1) , -- direct RNA sequencing
rna-size (2) , -- RNA length measurement
np-map (3) , -- nuclease protection mapping with homologous sequence ladder
np-size (4) , -- nuclease protected fragment length measurement
pe-seq (5) , -- dideoxy RNA sequencing
cDNA-seq (6) , -- full-length cDNA sequencing
pe-map (7) , -- primer extension mapping with homologous sequence ladder
pe-size (8) , -- primer extension product length measurement
pseudo-seq (9) , -- full-length processed pseudogene sequencing
rev-pe-map (10) , -- see NOTE (1) below
other (255) } ,
expression-system ENUMERATED {
unknown (0) ,
physiological (1) ,
in-vitro (2) ,
oocyte (3) ,
transfection (4) ,
transgenic (5) ,
other (255) } DEFAULT physiological ,
low-prec-data BOOLEAN DEFAULT FALSE ,
from-homolog BOOLEAN DEFAULT FALSE } -- experiment actually done on
-- close homolog
-- NOTE (1) length measurement of a reverse direction primer-extension
-- product (blocked by RNA 5'end) by comparison with
-- homologous sequence ladder (J. Mol. Biol. 199, 587)
END
0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0013 0014 0015 0016 0017 0018 0019 0020 0021 0022 0023 0024 0025 0026 0027 0028 0029 0030 0031 0032 0033 0034 0035 0036 0037 0038 0039 0040 0041 0042 0043 0044 0045 0046 0047 0048 0049 0050 0051 0052 0053 0054 0055 0056 0057 0058 0059 0060 0061 0062 0063 0064 0065 0066 0067 0068 0069 0070 0071 0072 0073 0074 0075 0076 0077 0078 0079 0080 0081 0082 0083 0084 0085 0086 0087 0088 0089 0090 0091 0092 0093 0094 0095 0096 0097 0098 0099 0100 0101 0102 0103 0104 0105 0106 0107 0108 0109 0110 0111 0112 0113 0114 0115 0116 0117 0118 0119 0120 0121 0122 0123 0124 0125 0126 0127 0128 0129 0130 0131 0132 0133 0134 0135 0136 0137 0138 0139 0140 0141 0142 0143 0144 0145 0146 0147 0148 0149 0150 0151 0152 0153 0154 0155 0156 0157 0158 0159 0160 0161 0162 0163 0164 0165 0166 0167 0168 0169 0170 0171 0172 0173 0174 0175 0176 0177 0178 0179 0180 0181 0182 0183 0184 0185 0186 0187 0188 0189 0190 0191 0192 0193 0194 0195 0196 0197 0198 0199 0200 0201 0202 0203 0204 0205 0206 0207 0208 0209 0210 0211 0212 0213 0214 0215 0216 0217 0218 0219 0220 0221 0222 0223 0224 0225 0226 0227 0228 0229 0230 0231 0232 0233 0234 0235 0236 0237 0238 0239 0240 0241 0242 0243 0244 0245 0246 0247 0248 0249 0250 0251 0252 0253 0254 0255 0256 0257 0258 0259 0260 0261 0262 0263 0264 0265 0266 0267 0268 0269 0270 0271 0272 0273 0274 0275 0276 0277 0278 0279 0280 0281 0282 0283 0284 0285 0286 0287 0288 0289 0290 0291 0292 0293 0294 0295 0296 0297 0298 0299 0300 0301 0302 0303 0304 0305 0306 0307 0308 0309 0310 0311 0312 0313 0314 0315 0316 0317 0318 0319 0320 0321 0322 0323 0324 0325 0326 0327 0328 0329 0330 0331 0332 0333 0334 0335 0336 0337 0338 0339 0340 0341 0342 0343 0344 0345 0346 0347 0348 0349 0350 0351 0352 0353 0354 0355 0356 0357 0358 0359 0360 0361 0362 0363 0364 0365 0366 0367 0368 0369 0370 0371 0372 0373 0374 0375 0376 0377 0378 0379 0380 0381 0382 0383 0384 0385 0386 0387 0388 0389 0390 0391 0392 0393 0394 0395 0396 0397 0398 0399 0400 0401 0402 0403 0404 0405 0406 0407 0408 0409 0410 0411 0412 0413 0414 0415 0416 0417 0418 0419 0420 0421 0422 0423 0424 0425 0426 0427 0428 0429 0430 0431 0432 0433 0434 0435 0436 0437 0438 0439 0440 0441 0442 0443 0444 0445 0446 0447 0448 0449 0450 0451 0452 0453 0454 0455 0456 0457 0458 0459 0460 0461 0462 0463 0464 0465 0466 0467 0468 0469 0470 0471 0472 0473 0474 0475 0476 0477 0478 0479 0480 0481 0482 0483 0484 0485 0486 0487 0488 0489 0490 0491 0492 0493 0494 0495 0496 0497 0498 0499 0500 0501 0502 0503 0504 0505 0506 0507 0508 0509 0510 0511 0512 0513 0514 0515 0516 0517 0518 0519 0520 0521 0522 0523 0524 0525 0526 0527 0528 0529 0530 0531 0532 0533 0534 0535 0536 0537 0538 0539 0540 0541 0542 0543 0544 0545 0546 0547 0548 0549 0550 0551 0552 0553 0554 0555 0556 0557 0558 0559 0560 0561 0562 0563 0564 0565 0566 0567 0568 0569 0570 0571 0572 0573 0574 0575 0576 0577 0578 0579 0580 0581 0582 0583 0584 0585 0586 0587 0588 0589 0590 0591 0592 0593 0594 0595 0596 0597 0598 0599 0600 0601 0602 0603 0604 0605 0606 0607 0608 0609 0610 0611 0612 0613 0614 0615 0616 0617 0618 0619 0620 0621 0622 0623 0624 0625 0626 0627 0628 0629 0630 0631 0632 0633 0634 0635 0636 0637 0638 0639 0640 0641 0642 0643 0644 0645 0646 0647 0648 0649 0650 0651 0652 0653 0654 0655 0656 0657 0658 0659 0660 0661 0662 0663 0664 0665 0666 0667 0668 0669 0670 0671 0672 0673 0674 0675 0676 0677 0678 0679 0680 0681 0682 0683 0684 0685 0686 0687 0688 0689 0690 0691 0692 0693 0694 0695 0696 0697 0698 0699 0700 0701 0702 0703 0704 0705 0706 0707 0708 0709 0710 0711 0712 0713 0714 0715 0716 0717 0718 0719 0720 0721 0722 0723 0724 0725 0726 0727 0728 0729 0730 0731 0732 0733 0734 0735 0736 0737 0738 0739 0740 0741 0742 0743 0744 0745 0746 0747 0748 0749 0750 0751 0752 0753 0754 0755 0756 0757 0758 0759 0760 0761 0762 0763 0764 0765 0766 0767 0768 0769 0770 0771 0772 0773 0774 0775 0776 0777 0778 0779 0780 0781 0782 0783 0784 0785 0786 0787 0788 0789 0790 0791 0792 0793 0794 0795 0796 0797 0798 0799 0800 0801 0802 0803 0804 0805 0806 0807 0808 0809 0810 0811 0812 0813 0814 0815 0816 0817 0818 0819 0820 0821 0822 0823 0824 0825 0826 0827 0828 0829 0830 0831 0832 0833 0834 0835 0836 0837 0838 0839 0840 0841 0842 0843 0844 0845 0846 0847 0848 0849 0850 0851 0852 0853 0854 0855 0856 0857 0858 0859 0860 0861 0862 0863 0864 0865 0866 0867 0868 0869 0870 0871 0872 0873 0874 0875 0876 0877 0878 0879 0880 0881 0882 0883 0884 0885 0886 0887 0888 0889 0890 0891 0892 0893 0894 0895 0896 0897 0898 0899 0900 0901 0902 0903 0904 0905 0906 0907 0908 0909 0910 0911 0912 0913 0914 0915 0916 0917 0918 0919 0920 0921 0922 0923 0924 0925 0926 0927 0928 0929 0930 0931 0932 0933 0934 0935 0936 0937 0938 0939 0940 0941 0942 0943 0944 0945 0946 0947 0948 0949 0950 0951 0952 0953 0954 0955 0956 0957 0958 0959 0960 0961 0962 0963 0964 0965 0966 0967 0968 0969 0970 0971 0972 0973 0974 0975 0976 0977 0978 0979 0980 0981 0982 0983 0984 0985 0986 0987 0988 0989 0990 0991 0992 0993 0994 0995 0996 0997 0998 0999 1000 1001 1002 1003 1004 1005 1006 1007 1008 1009 1010 1011 1012 1013 1014 1015 1016 1017 1018 1019 1020 1021 1022 1023 1024 1025 1026 1027 1028 1029 1030 1031 1032 1033 1034 1035 1036 1037 1038 1039 1040 1041 1042 1043 1044 1045 1046 1047 1048 1049 1050 1051 1052 1053 1054 1055 1056 1057 1058 1059 1060 1061 1062 1063 1064 1065 1066 1067 1068 1069 1070 1071 1072 1073 1074 1075 1076 1077 1078 1079 1080 1081 1082 1083 1084 1085 1086 1087 1088 1089 1090 1091 1092 1093 1094 1095 1096 1097 1098 1099 1100 1101 1102 1103 1104 1105 1106 1107 1108 1109 1110 1111 1112 1113 1114 1115 1116 1117 1118 1119 1120 1121 1122 1123 1124 1125 1126 1127 1128 1129 1130 1131 1132 1133 1134 1135 1136 1137 1138 1139 1140 1141 1142 1143 1144 1145 1146 1147 1148 1149 1150 1151 1152 1153 1154 1155 1156 1157 1158 1159 1160 1161 1162 1163 1164 1165 1166 1167 1168 1169 1170 1171 1172 1173 1174 1175 1176 1177 1178 1179 1180 1181 1182 1183 1184 1185 1186 1187 1188 1189 1190 1191 1192 1193 1194 1195 1196 1197 1198 1199 1200 1201 1202 1203 1204 1205 1206 1207 1208 1209 1210 1211 1212 1213 1214 1215 1216 1217 1218 1219 1220 1221 1222 1223 1224 1225 1226 1227 1228 1229 1230 1231 1232 1233 1234 1235 1236 1237 1238 1239 1240 1241 1242 1243 1244 1245 1246 1247 1248 1249 1250 1251 1252 1253 1254 1255 1256 1257 1258 1259 1260 1261 1262 1263 1264 1265 1266 1267 1268 1269 1270 1271 1272 1273 1274 1275 1276 1277 1278 1279 1280 1281 1282 1283 1284 1285 1286 1287 1288 1289 1290 1291 1292 1293 1294 1295 1296 1297 1298 1299 1300 1301 1302 1303 1304 1305 1306 1307 1308 1309 1310 1311 1312 1313 1314 1315 1316 1317 1318 1319 1320 1321 1322 1323 1324 1325 1326 1327 1328 1329 1330 1331 1332 1333 1334 1335 1336 1337 1338 1339 1340 1341 1342 1343 1344 1345 1346 1347 1348 1349 1350 1351 1352 1353 1354 1355 1356 1357 1358 1359 1360 1361 1362 1363 1364 1365 1366 1367 1368 1369 1370 1371 1372 1373 1374 1375 1376 1377 1378 1379 1380 1381 1382 1383 1384 1385 1386 1387 1388 1389 1390 1391 1392 1393 1394 1395 1396 1397