Listeria monocytogenes Strain R2-502 - SRA

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SRX320106: Listeria monocytogenes Strain R2-502
8 PACBIO_SMRT (PacBio RS) runs: 653,928 spots, 1.5G bases, 5.6Gb downloads

Submitted by: UCD-100K

Study: Major food bacterial pathogens in the united states and around the world, including Salmonella enterica, E. coli, Shigella, Listeria monocytogenes, Campylobacter Jejuni, and Vibrio parahaemolyticus genome sequencing

PRJNA203445 • SRP022914 • All experiments • All runs

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University of California at Davis 100K Pathogen Genome Project. Major bacterial pathogens in the United States and around the world, including Salmonella enterica, E. coli, Shigella, Listeria monocytogenes, Campylobacter Jejuni, Vibrio parahaemolyticus. This project also contains WGS from many food-associated organisms that have unique traits.

Sample: Pathogen sample from Listeria monocytogenes

SAMN02203126 • SRS456501 • All experiments • All runs

Organism: Listeria monocytogenes

Library:

Instrument: PacBio RS

Strategy: WGS

Source: GENOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: SMRTbell sequencing libraries were generated using standard procedures (version C2; http://www.pacb.com/samplenet/PC_10kb_Template_Preparation_and_Sequencing.pdf). gDNA was sheared to an average size of approximately 10 kb using g-TUBEs (Covaris). Fragmented DNA underwent DNA Damage Repair, End Repair, and blunt hairpin adapters were ligated onto each end. Incompletely formed SMRTbell templates were degraded with a combination of Exo III and Exo VII. SMRTbell templates were magbead loaded and sequenced with C2 chemistry.

Spot descriptor:

1 forward

Runs: 8 runs, 653,928 spots, 1.5G bases, 5.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR932060	81,741	178.7M	700.3Mb	2013-07-12
SRR932061	81,741	157.3M	622.9Mb	2013-07-12
SRR932062	81,741	162.6M	642.6Mb	2013-07-12
SRR932063	81,741	181M	710.8Mb	2013-07-12
SRR932064	81,741	167.1M	648.4Mb	2013-07-12
SRR932065	81,741	144.6M	566.9Mb	2013-07-12
SRR932066	81,741	248.3M	931.7Mb	2013-07-12
SRR932067	81,741	248.8M	933.8Mb	2013-07-12

ID:: 447175

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