Illumina HiSeq 2000 paired end sequencing; Transcriptomic signatures ... - SRA

ERX446086: Illumina HiSeq 2000 paired end sequencing; Transcriptomic signatures of cartilage ageing
1 ILLUMINA (Illumina HiSeq 2000) run: 30.9M spots, 6.2G bases, 3.7Gb downloads

Design: Transcriptomic signatures of cartilage ageing

Submitted by: UNIVERSITY OF LIVERPOOL

Study: RNA-seq of achilles tendon from young and old donors to study age-related changes in tendon

PRJEB6129 • ERP005598 • All experiments • All runs

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Tendon from young and old donors was used for RNA-Seq analysis. The aim of the study was to identify differentially expressed tendon transcripts in ageing in order to to characterize molecular mechanisms associated with age-related changes in tendon.

Sample: Young3

SAMEA2467149 • ERS436906 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: ex_Young3

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Construction protocol: Full thickness achilles tendon was removed from the mid tendon. All samples were grossly normal. For RNA extraction the Guanidium-thiocyanate-phenol-chloroform technique, with ethanol extraction was used (Chomczynski, P. and N. Sacchi, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem, 1987

Spot descriptor:

1 forward101 reverse

Experiment attributes:

Experimental Factor: age: 27 year

LIBRARY_STRAND: second strand

Runs: 1 run, 30.9M spots, 6.2G bases, 3.7Gb

Run	# of Spots	# of Bases	Size	Published
ERR480254	30,907,574	6.2G	3.7Gb	2015-04-02

ID:: 1420314

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