Illumina HiSeq 2000 paired end sequencing; Transcriptomic signatures ... - SRA

ERX446081: Illumina HiSeq 2000 paired end sequencing; Transcriptomic signatures of cartilage ageing
1 ILLUMINA (Illumina HiSeq 2000) run: 37.5M spots, 7.5G bases, 4.4Gb downloads

Design: Transcriptomic signatures of cartilage ageing

Submitted by: UNIVERSITY OF LIVERPOOL

Study: RNA-seq of achilles tendon from young and old donors to study age-related changes in tendon

PRJEB6129 • ERP005598 • All experiments • All runs

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Tendon from young and old donors was used for RNA-Seq analysis. The aim of the study was to identify differentially expressed tendon transcripts in ageing in order to to characterize molecular mechanisms associated with age-related changes in tendon.

Sample: Young2

SAMEA2467144 • ERS436901 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: ex_Young2

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Construction protocol: Full thickness achilles tendon was removed from the mid tendon. All samples were grossly normal. For RNA extraction the Guanidium-thiocyanate-phenol-chloroform technique, with ethanol extraction was used (Chomczynski, P. and N. Sacchi, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem, 1987

Spot descriptor:

1 forward101 reverse

Experiment attributes:

Experimental Factor: age: 16 year

LIBRARY_STRAND: second strand

Runs: 1 run, 37.5M spots, 7.5G bases, 4.4Gb

Run	# of Spots	# of Bases	Size	Published
ERR480251	37,503,882	7.5G	4.4Gb	2015-04-02

ID:: 1420309

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