Abstract
A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increased resistance to cefoxitin were identified. Isoelectric focusing detected an M. tuberculosis-derived beta-lactamase in one of these recombinant clones. A sequence analysis identified it as a class A beta-lactamase whose expression correlated with the increased resistance phenotype.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Cefoxitin / pharmacology
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Cephamycins / pharmacology
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Cloning, Molecular
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Cosmids
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Isoelectric Focusing
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Molecular Sequence Data
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Mycobacterium tuberculosis / enzymology*
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Mycobacterium tuberculosis / genetics
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Sequence Analysis
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beta-Lactamases / classification
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beta-Lactamases / drug effects
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beta-Lactamases / genetics*
Substances
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Cephamycins
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Cefoxitin
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beta-Lactamases