New Macrolide-Lincosamide-Streptogramin B Resistance Gene erm(48) on the Novel Plasmid pJW2311 in Staphylococcus xylosus

Antimicrob Agents Chemother. 2017 Jun 27;61(7):e00066-17. doi: 10.1128/AAC.00066-17. Print 2017 Jul.

Abstract

Whole-genome sequencing of Staphylococcus xylosus strain JW2311 from bovine mastitis milk identified the novel 49.3-kb macrolide-lincosamide-streptogramin B (MLSB) resistance plasmid pJW2311. It contained the macrolide resistance gene mph(C), the macrolide-streptogramin B resistance gene msr(A), and the new MLSB resistance gene erm(48) and could be transformed into Staphylococcus aureus by electroporation. Functionality of erm(48) was demonstrated by cloning and expression in S. aureus.

Keywords: Staphylococcus; animal; antibiotic resistance; bovine milk; macrolide; macrolide-lincosamide-streptogramin B; plasmid-mediated resistance; rRNA methylase.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Drug Resistance, Multiple, Bacterial / genetics
  • Lincosamides / pharmacology*
  • Macrolides / pharmacology*
  • Microbial Sensitivity Tests
  • Plasmids / genetics*
  • Staphylococcus / drug effects*
  • Streptogramin B / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Lincosamides
  • Macrolides
  • Streptogramin B