Identification and characterization of genes underlying chitinolysis in Collimonas fungivorans Ter331

FEMS Microbiol Ecol. 2008 Oct;66(1):123-35. doi: 10.1111/j.1574-6941.2008.00547.x. Epub 2008 Jul 30.

Abstract

Through a combinatorial approach of plasposon mutagenesis, genome mining, and heterologous expression, we identified genes contributing to the chitinolytic phenotype of bacterium Collimonas fungivorans Ter331. One of five mutants with abolished ability to hydrolyze colloidal chitin carried its plasposon in the chiI gene coding for an extracellular endochitinase. Two mutants were affected in the promoter of chiP-II coding for an outer-membrane transporter of chitooligosaccharides. The remaining two mutations were linked to chitobiose/N-acetylglucosamine uptake. Thus, our model for the Collimonas chitinolytic system assumes a positive feedback regulation of chitinase activity by chitin degradation products. A second chitinase gene, chiII, coded for an exochitinase that preferentially released chitobiose from chitin analogs. Genes hexI and hexII showed coding resemblance to N-acetylglucosaminidases, and the activity of purified HexI protein towards chitin analogs suggested its role in converting chitobiose to N-acetylglucosamine. The hexI gene clustered with chiI, chiII, and chiP-II in one locus, while chitobiose/N-acetylglucosamine uptake genes colocalized in another. Both loci contained genes for conversion of N-acetylglucosamine to fructose-6-phosphate, confirming that C. fungivorans Ter331 features a complete chitin pathway. No link could be established between chitinolysis and antifungal activity of C. fungivorans Ter331, suggesting that the bacterium's reported antagonism towards fungi relies on other mechanisms.

MeSH terms

  • Acetylglucosamine / metabolism
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Chitin / metabolism*
  • Chitinases / genetics*
  • DNA, Bacterial / genetics
  • Disaccharides / metabolism
  • Fructosephosphates / metabolism
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial
  • Genome, Bacterial
  • Hexosaminidases / genetics*
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Oxalobacteraceae / genetics*
  • Promoter Regions, Genetic
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Disaccharides
  • Fructosephosphates
  • Chitin
  • chitobiose
  • fructose-6-phosphate
  • 1,4-beta-chitobiosidase
  • Hexosaminidases
  • Chitinases
  • Acetylglucosamine