ISEcp1-mediated transposition of qnrB-like gene in Escherichia coli

Vincent Cattoir; Patrice Nordmann; Jesus Silva-Sanchez; Paula Espinal; Laurent Poirel

doi:10.1128/AAC.00349-08

ISEcp1-mediated transposition of qnrB-like gene in Escherichia coli

Antimicrob Agents Chemother. 2008 Aug;52(8):2929-32. doi: 10.1128/AAC.00349-08. Epub 2008 Jun 2.

Authors

Vincent Cattoir¹, Patrice Nordmann, Jesus Silva-Sanchez, Paula Espinal, Laurent Poirel

Affiliation

¹ Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, K.-Bicêtre, France.

Abstract

A novel QnrB-like plasmid-mediated resistance determinant, QnrB19, was identified from an Escherichia coli clinical isolate from Colombia. Its gene was associated with an ISEcp1-like insertion element that did not act as a promoter for its expression. Using an in vitro model of transposition, we showed that the ISEcp1-like element was able to mobilize the qnrB19 gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA Transposable Elements / genetics*
Drug Resistance, Bacterial / genetics
Escherichia coli / drug effects
Escherichia coli / genetics*
Escherichia coli Proteins / genetics*
Microbial Sensitivity Tests
Molecular Sequence Data
Quinolones / pharmacology

Substances

DNA Transposable Elements
Escherichia coli Proteins
Quinolones

Associated data

GENBANK/EU432277
GENBANK/EU523120